Cat.No. : CSC-RG0012 Host Cell: U2OS
| Cat. No. | CSC-RG0012 |
| Background | Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins. The alpha-adrenergic receptor (AR) subtypes (alpha(1a), alpha(1b), and alpha(1d)) mediate some physiological effects of epinephrine and norepinephrine. The alpha1-AR subtypes are expressed in several organs like brain or heart in which they modulate a diversity of functional effects such as modulation of neurotransmission, vasoconstriction, etc. |
| Gene | ADRA1A |
| Gene Species | Homo sapiens (Human) |
| Alias | ADRA1A, ADRA1C, ADRA1L1, ALPHA1AAR |
| Host Cell | U2OS |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human ADRA1A Stable Cell Line-U2OS, established by stably expressing the human α1A adrenergic receptor (ADRA1A) in U2OS cells, is crucial for studying how adrenergic receptors regulate intracellular signaling and physiological responses. It aids scientists in understanding the role of ADRA1A in the cardiovascular system, nervous system, and stress responses.
A: Drug screening on the Human ADRA1A Stable Cell Line-U2OS allows researchers to evaluate the affinity and effects of potential drugs on ADRA1A. This cell line provides a platform for testing the impact of drugs on ADRA1A signaling, which is significant for developing drugs to treat cardiovascular diseases.
A: The Human ADRA1A Stable Cell Line-U2OS allows scientists to investigate how ADRA1A gene mutations affect cellular function. By comparing the expression and function of wild-type and mutant ADRA1A, researchers can better understand how these mutations lead to changes in cellular signal transduction, which is significant for the study of genetic cardiovascular diseases.
A: The Human ADRA1A Stable Cell Line-U2OS can be used to study the role of ADRA1A in stress responses, particularly in dealing with environmental stressors and physiological challenges. This cell line helps scientists understand the molecular mechanisms of ADRA1A in regulating stress responses, which has potential value for developing treatment strategies for stress-related diseases.
A: The Human ADRA1A Stable Cell Line-U2OS provides an experimental model for studying the role of ADRA1A in cardiovascular diseases. Through this cell line, scientists can investigate how ADRA1A affects the function of the heart and blood vessels, which is crucial for understanding the pathogenesis of cardiovascular diseases and developing new treatment strategies.
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