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rAAV-hSyn-Con/Fon-TVA-mCherry

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-30

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Virus Particles Information

Cat. No. RABVH-30
Description Neuron-specific AAV helper using a Con/Fon (Cre-ON AND Flp-ON) Boolean cassette to express a TVA-mCherry fusion under hSyn. Only neurons containing both recombinases become permissive to EnvA-pseudotyped G-deleted rabies virus and are labelled red. With RVG, this enables dual-recombinase intersectional retrograde monosynaptic input mapping in neurons.
Product Type Adeno-associated virus
Reporter mCherry
Expression Mechanism Boolean Algebra
Helper Function AAV helper for Cre AND Flp intersectional TVA in neurons
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre AND Flp intersectional monosynaptic retrograde tracing in neurons
  2. Con/Fon expresses TVA only when BOTH Cre and Flp are present
  3. hSyn restricts to neurons
  4. mCherry fused with TVA for visualization
  5. Mapping direct presynaptic inputs to intersectional neuronal populations
Promoter Neuron-specific Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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