Pages
Products

rAAV-EF1a-Coff/Fon-H2B-EGFP-P2A-TVA

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-25

Inquire for Price

Virus Particles Information

Cat. No. RABVH-25
Description AAV helper carrying a Coff/Fon (Cre-OFF AND Flp-ON) Boolean cassette that drives nuclear H2B-EGFP and TVA via P2A. TVA expression is confined to neurons that are Flp-positive but Cre-negative, so EnvA-pseudotyped G-deleted rabies virus initiates only in that subtractive population. With RVG, this supports Cre-OFF/Flp-ON monosynaptic retrograde mapping.
Product Type Adeno-associated virus
Reporter EGFP
Expression Mechanism Boolean Algebra
Helper Function AAV helper for Cre-OFF Flp-ON Boolean TVA expression
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre OFF Flp ON monosynaptic retrograde tracing
  2. Coff/Fon expresses TVA when Flp is present AND Cre is absent
  3. Nuclear-localized EGFP reporter
  4. Subtractive genetic targeting
  5. Mapping direct presynaptic inputs to Flp+ but Cre- populations
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
Quick Inquiry

Q & A

Customer Reviews

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Needs improvement

Satisfaction

General satisfaction

Very satisfaction