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rAAV-EF1a-fDIO-TVA-EGFP

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-3

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Virus Particles Information

Cat. No. RABVH-3
Description Flp-dependent AAV helper expressing a TVA-EGFP fusion under EF1a. TVA confers selective susceptibility to EnvA-pseudotyped G-deleted rabies virus on Flp-expressing neurons, while EGFP labels these starter cells in green. Combined with RVG complementation, the virus spreads retrogradely across one synapse to reveal direct presynaptic inputs of the Flp-defined population.
Product Type Adeno-associated virus
Reporter EGFP
Expression Mechanism Flp-dependent
Helper Function AAV helper co-expressing TVA and EGFP under Flp-dependent control (fusion)
Applications
  1. Co-injection with RV-EnvA-ΔG for Flp-dependent monosynaptic retrograde tracing with green starter labeling
  2. EGFP fused with TVA for direct visualization of TVA-expressing cells
  3. Single AAV vector for simplified injection protocol
  4. fDIO restricts expression to Flp+ populations
  5. Mapping direct presynaptic inputs to Flp-defined populations
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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