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rAAV-EF1a-DIO-NLS-BFP-F2A-TVA-T2A-RVG

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-53

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Virus Particles Information

Cat. No. RABVH-53
Description Cre-dependent all-in-one AAV helper co-expressing nuclear BFP, TVA and RVG via F2A/T2A under EF1a. TVA allows EnvA-pseudotyped G-deleted rabies virus to enter Cre-positive neurons, and RVG supplied from the same cassette complements the G deletion for one-step retrograde spread. Nuclear BFP marks starter cells while leaving green and red channels free.
Product Type Adeno-associated virus
Reporter BFP
Expression Mechanism Cre-dependent
Helper Function AAV helper co-expressing nuclear BFP, TVA, and RVG via F2A/T2A under Cre-dependent control
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre-dependent monosynaptic retrograde tracing with nuclear blue starter
  2. NLS-BFP for nuclear starter visualization
  3. F2A and T2A for tricistronic expression
  4. DIO restricts all components to Cre+ populations
  5. Triple-color experiments with EGFP and mCherry RV tracers
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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