Pages
Products

rAAV-EF1a-Con/Foff-H2B-EGFP-P2A-TVA

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-24

Inquire for Price

Virus Particles Information

Cat. No. RABVH-24
Description AAV helper with a Con/Foff Boolean cassette expressing nuclear H2B-EGFP and TVA via P2A. TVA is produced only in neurons that are Cre-positive but Flp-negative, restricting entry of EnvA-pseudotyped G-deleted rabies virus to this subtractive population. Combined with an RVG helper, it enables Cre-ON/Flp-OFF intersectional monosynaptic retrograde tracing.
Product Type Adeno-associated virus
Reporter EGFP
Expression Mechanism Boolean Algebra
Helper Function AAV helper for Cre-ON Flp-OFF Boolean TVA expression
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre ON Flp OFF monosynaptic retrograde tracing
  2. Con/Foff expresses TVA when Cre is present AND Flp is absent
  3. Nuclear-localized EGFP reporter
  4. Subtractive genetic targeting
  5. Mapping direct presynaptic inputs to Cre+ but Flp- populations
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
Quick Inquiry

Q & A

Customer Reviews

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Needs improvement

Satisfaction

General satisfaction

Very satisfaction