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rAAV-CaMKIIa-EGFP-T2A-TVA

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-34

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Virus Particles Information

Cat. No. RABVH-34
Description Constitutive AAV helper co-expressing cytoplasmic EGFP and TVA via T2A under CaMKIIa, targeting excitatory neurons. EGFP labels the transduced starter cells green, and TVA makes them selectively permissive to EnvA-pseudotyped G-deleted rabies virus. When supplemented with RVG, the virus crosses one synapse retrogradely, revealing direct inputs to excitatory neurons.
Product Type Adeno-associated virus
Reporter EGFP
Helper Function AAV helper expressing EGFP and TVA in excitatory neurons
Applications
  1. Co-injection with RV-EnvA-ΔG for constitutive monosynaptic retrograde tracing in excitatory neurons
  2. CaMKIIα restricts to excitatory pyramidal neurons
  3. EGFP and TVA co-expressed via T2A
  4. Mapping direct presynaptic inputs to excitatory projection neurons
  5. Standard monosynaptic retrograde tracing in cortical/hippocampal excitatory circuits
Promoter Neuron-specific Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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