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Cat.No. : CSC-RO01309 Host Cell: Human urinary bladder carcinoma cell line (T24)
| Cat. No. | CSC-RO01309 |
| Description | T24-Human-TACSTD2 is engineered to stably overexpress human tumor associated calcium signal transducer 2 (TACSTD2, Trop 2, NM_002353.2). The overexpression of human TACSTD2 has been validated by real-time qPCR and flow cytometry analysis. |
| Background | TACSTD2 is a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of TACSTD2 gene have been associated with gelatinous drop-like corneal dystrophy. |
| Gene | TACSTD2 |
| Gene Species | Homo sapiens (human) |
| Host Cell | Human urinary bladder carcinoma cell line (T24) |
| Host Cell Species | Homo sapiens (Human) |
| Stability | This cell line is stable at least 10 passages. |
| Product Type | Human gene overexpression stable cell line |
| Applications | 1) investigation of gene function 2) screening and validation of antibodies 3) anticancer drug development |
| Quality Control | 1) Real-time qPCR analysis of gene mRNA overexpression level 2) Flow cytometry analysis of gene surface overexpression level 3) mycoplasma detection |
| Size Form | One vial of frozen cells, typically >1x10^6cells/vial |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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