Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO01309
Host Cell : T24 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO01309 |
| Description | T24-Human-TACSTD2 is engineered to stably overexpress human tumor associated calcium signal transducer 2 (TACSTD2, Trop 2, NM_002353.2). The overexpression of human TACSTD2 has been validated by real-time qPCR and flow cytometry analysis. |
| Product Type | Human gene overexpression stable cell line |
| Background | TACSTD2 is a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of TACSTD2 gene have been associated with gelatinous drop-like corneal dystrophy. |
| Target Gene | TACSTD2 |
| Gene Species | Homo sapiens (human) |
| Host Cell | T24 |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1) investigation of gene function 2) screening and validation of antibodies 3) anticancer drug development |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) Real-time qPCR analysis of gene mRNA overexpression level 2) Flow cytometry analysis of gene surface overexpression level 3) mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | TACSTD2 |
| Background | This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy.[provided by RefSeq, Dec 2009] |
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
We purchased the Human TACSTD2 (Trop2) Stable Cell Line in the T24 bladder cancer background for our antibody-drug conjugate (ADC) development project, and it has exceeded our expectations. Trop2 is overexpressed in many epithelial cancers, and having this stable cell line allowed us to evaluate target binding, internalization, and cytotoxicity of our Trop2-directed ADCs in a highly relevant cellular context.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.