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Human CEACAM5 Stable Cell Line - PC-3

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO01341

Host Cell :   PC3 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RO01341
Description This cell line is engineered to stably express Homo sapiens (human) CEA cell adhesion molecule 5 (CEACAM5) in Human prostatic adenocarcinoma / prostate cancer cell line (PC-3). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells.
Product Type Human gene overexpression stable cell line
Target Gene CEACAM5
Gene Species Homo sapiens (human)
Host Cell PC3
Host Cell Species Homo sapiens (Human)
Reporter GFP
Applications 1) investigation of gene function
2) screening and validation of antibodies
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) Real-time qPCR analysis of gene mRNA overexpression level
2) GFP fluorescent detection under fluorescent microscopy
3) mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene CEACAM5
Background This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
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Work well

The Human CEACAM5 Stable Cell Line - PC-3 offered strong and consistent expression levels, making it ideal for our tumor marker investigations. We were impressed by the product quality and technical support.

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