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Human CEACAM5 Stable Cell Line - CT26

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO01307

Host Cell :   CT26 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Documents

Cat. No. CSC-RO01307
Description This cell line is engineered to stably express human CEACAM5 in CT26 cells.
Product Type Stable cell line constitutively expressing ion channel gene(s)
Target Gene CEACAM5
Gene Species Homo sapiens (Human)
Host Cell CT26
Host Cell Species Mus musculus (Mouse)
Applications 1) in vitro cell-based assays
2) in vivo tumor growth studies
Resistance G418
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) Real-Time qPCR
2) Flow cytometry analysis
3) Mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene CEACAM5
Background This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
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