Cat.No. : CSC-RO0246 Host Cell: HEK293T
| Cat. No. | CSC-RO0246 |
| Description | This cell line stably expresses cynomolgus LAG3 gene. |
| Gene | LAG3 |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Gene Name | LAG3 lymphocyte-activation gene 3 [ Homo sapiens ] |
| Gene Symbol | LAG3 |
| Synonyms | CD223 |
| Gene Description | lymphocyte-activation gene 3 |
| Gene ID | 3902 |
| UniProt ID | P18627 |
| mRNA Refseq | NM_002286.5 |
| Protein Refseq | NP_002277.4 |
| Chromosome Location | 12p13.32 |
| Function | MHC class II protein binding; antigen binding; transmembrane signaling receptor activity; |
| MIM | 153337 |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Monkey LAG3 Stable Cell Line-HEK293T plays a crucial role in the study of immune checkpoint inhibitors by providing a platform to investigate the function of LAG3 (Lymphocyte Activation Gene 3), a protein that acts as a negative regulator of T cell activation. This cell line can be used to study the interaction between LAG3 and other immune checkpoint molecules, contributing to the development of therapeutic strategies for cancer and autoimmune diseases.
A: The Monkey LAG3 Stable Cell Line-HEK293T is derived from human embryonic kidney cells (HEK293T), which are known for their high transfection efficiency and ability to express foreign proteins at high levels. This makes them an ideal choice for stable cell line generation, especially for studying proteins like LAG3 that are involved in complex immune regulatory processes.
A: The generation of a stable cell line expressing LAG3 in HEK293T cells can be challenging due to the need for efficient gene transfer, selection, and validation. This process requires optimizing transfection protocols, developing appropriate selection markers, and ensuring the stable and functional expression of LAG3 without compromising cell viability or growth.
A: The cell line can be used in high-throughput screening assays to identify compounds that modulate LAG3 activity, which could potentially lead to the development of new immunotherapies. By testing the effects of various compounds on LAG3 signaling pathways, researchers can identify potential drug candidates that could enhance immune responses or suppress autoimmunity.
A: The cell line allows researchers to model the cellular responses to LAG3, which is often overexpressed in cancer cells to evade immune detection. By studying the LAG3 signaling pathway in this context, scientists can better understand the molecular mechanisms underlying immune evasion and develop targeted therapies to enhance anti-tumor immunity.
A: The cell line can be used in co-immunoprecipitation experiments, immunofluorescence assays, and other techniques to investigate the protein-protein interactions involving LAG3. This can help elucidate the complex network of interactions that regulate immune responses.
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