Cat.No. : CSC-RO0107 Host Cell: HEK293T
| Cat. No. | CSC-RO0107 |
| Description | HEK293T-LAG3 cell line is a stably transfected cell line which expresses human lymphocyte activating 3 (LAG3). |
| Gene | LAG3 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The researchers used the Human LAG3 Stable Cell Line-HEK293T to study the LAG3 protein's role in immune response modulation. LAG3 is a transmembrane protein that inhibits cell activation and proliferation, resulting in optimal immune response modulation. Using in vitro phage display technique, scientists discovered a new single-chain variable fragment (scFv) antibody that targets LAG3. This antibody was reconstituted as an IgG and evaluated for binding specificity. They used antigen-specific CD8+ T lymphocyte clones to show that the antibody increases cell activation, implying that it could be used as an immunological checkpoint inhibitor in cancer therapy.
Figure 1. The researchers investigated the binding properties of the divalent scFvF7-Fc antibody. They used ELISA to test binding to recombinant LAG3, FACS analysis on LAG3-overexpressing 293T cells, and dose-response binding on activated human CD8+ T cells. The results demonstrated that the antibody bound in a specific and dose-dependent manner. (Ascione A, et al., 2019)
A: The Human LAG3 Stable Cell Line-HEK293T is constructed by introducing the LAG3 (Lymphocyte Activation Gene 3) gene's encoding sequence into the HEK293T cell line using viral vector-mediated gene transfer techniques, such as retroviruses or lentiviruses, to ensure stable expression of the LAG3 gene in the cells. The construction of this cell line typically involves the use of selection markers, such as antibiotic resistance genes, to screen for cells that have successfully integrated and express the LAG3 gene.
A: The role of the Human LAG3 Stable Cell Line-HEK293T in studying immune regulation is to provide a model system for investigating the function of the LAG3 protein in immune cell activation, inhibition, and tolerance formation. This cell line helps to reveal the key role of LAG3 in regulating T cell responses and anti-tumor immunity.
A: The role of the Human LAG3 Stable Cell Line-HEK293T in studying LAG3-mediated immune suppression is to provide an experimental model for investigating how LAG3 regulates the activity of immune cells, particularly in suppressing T cell responses. This cell line helps researchers understand the mechanisms of LAG3 in immune tolerance and autoimmune diseases.
A: The application of the Human LAG3 Stable Cell Line-HEK293T in the development of LAG3-targeted therapies is as a screening platform to identify and validate compounds that can modulate LAG3 activity. These compounds may contribute to the development of new immunomodulatory drugs for the treatment of cancer and autoimmune diseases.
A: The role of the Human LAG3 Stable Cell Line-HEK293T in studying the interactions between LAG3 and other immune checkpoint molecules, such as PD-1 and CTLA-4, is to provide an experimental environment to investigate how these molecules synergistically act at the cellular level and how they collectively affect the function of immune cells. This is significant for developing combined immunotherapy strategies.
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The Human LAG3 Stable Cell Line-HEK293T is an ideal model for studying the role of LAG3 in immune regulation.
Provides a stable platform to study the function of the LAG3 gene and its role in cellular immunity.
Through this cell line, the relationship between LAG3 and autoimmune diseases and cancer immune evasion can be studied.
The Human LAG3 Stable Cell Line-HEK293T contributes to a deeper understanding of the role of LAG3 in immune evasion.
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