Human INSR Stable Cell Line - HEK293T

Name: Human INSR Stable Cell Line - HEK293T
SKU: CSC-RO0552
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0552

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0552
Description	This cell line is derived from HEK293T and is engineered to stably overexpress Human INSR.
Target Gene	INSR
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	INSR insulin receptor [ Homo sapiens ]
Gene Symbol	INSR
Synonyms	HHF5; CD220
Gene Description	insulin receptor
Gene ID	3643
Uni Prot ID	P06213
m RNA Refseq	NM_001079817.1
Protein Refseq	NP_001073285.1
Chromosome Location	19p13.3-p13.2
Function	3-phosphoinositide-dependent protein kinase binding; ATP binding; GTP binding; PTB domain binding; SH2 domain binding; insulin binding; insulin binding; insulin receptor substrate binding; insulin-activated receptor activity; insulin-like growth factor I binding; insulin-like growth factor II binding; insulin-like growth factor receptor binding; lipoic acid binding; phosphatidylinositol 3-kinase binding; protein binding; protein complex binding; protein phosphatase binding; protein tyrosine kinase activity; protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity;
Pathway	AMPK signaling, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; DNA damage response (only ATM dependent), organism-specific biosystem; HIF-1 signaling pathway, organism-specific biosystem;
MIM	147670

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Customer Q&As

What is the potential application of Human INSR Stable Cell Line - HEK293T in diabetes model research?

A: Human INSR Stable Cell Line - HEK293T, with its stable expression of insulin receptor (INSR), holds significant potential for application in diabetes model research. This cell line can be used to assess the effects of insulin analogs, study defects in insulin signaling pathways, and develop new diabetic treatments.

How can the molecular mechanisms of insulin resistance be studied using Human INSR Stable Cell Line - HEK293T?

A: Using Human INSR Stable Cell Line - HEK293T, the molecular mechanisms of insulin resistance can be studied through various methods, such as Western blotting to analyze key proteins in insulin signaling pathways or flow cytometry to assess the expression and function of insulin receptors on the cell surface.

How should experiments be designed for drug screening using Human INSR Stable Cell Line - HEK293T to ensure accuracy and reproducibility?

A: To ensure accuracy and reproducibility in drug screening, standardized culture conditions, strict control of cell density and culture duration should be employed when using Human INSR Stable Cell Line - HEK293T. Also, appropriate control groups and multiple replicates should be used to validate the effects of drugs.

What unique insights can Human INSR Stable Cell Line - HEK293T provide in obesity research?

A: Human INSR Stable Cell Line - HEK293T can provide unique insights into the role of insulin receptor function and insulin signaling in the development of obesity, especially in studying insulin resistance and metabolic disorders associated with obesity. This cell line is a valuable tool for such research.

What key factors should be considered when conducting gene expression analysis using Human INSR Stable Cell Line - HEK293T?

A: When conducting gene expression analysis, it is important to choose appropriate methods (such as real-time quantitative PCR or RNA sequencing) and strictly control experimental conditions to avoid technical biases. Additionally, the impact of insulin treatment on cellular gene expression and appropriate control experiments should be considered.

How can Human INSR Stable Cell Line - HEK293T be utilized in studying various aspects of metabolic syndrome?

A: Human INSR Stable Cell Line - HEK293T can play a role in studying various aspects of metabolic syndrome, including abnormalities in glucose metabolism, insulin resistance, and lipid metabolism disorders. This cell line can be used to evaluate the efficacy of drugs treating these conditions and to study the regulation of metabolic pathways.

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