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Cat.No. : CSC-RO0552 Host Cell: HEK293T
| Cat. No. | CSC-RO0552 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Human INSR. |
| Gene | INSR |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Human INSR Stable Cell Line - HEK293T, with its stable expression of insulin receptor (INSR), holds significant potential for application in diabetes model research. This cell line can be used to assess the effects of insulin analogs, study defects in insulin signaling pathways, and develop new diabetic treatments.
A: Using Human INSR Stable Cell Line - HEK293T, the molecular mechanisms of insulin resistance can be studied through various methods, such as Western blotting to analyze key proteins in insulin signaling pathways or flow cytometry to assess the expression and function of insulin receptors on the cell surface.
A: To ensure accuracy and reproducibility in drug screening, standardized culture conditions, strict control of cell density and culture duration should be employed when using Human INSR Stable Cell Line - HEK293T. Also, appropriate control groups and multiple replicates should be used to validate the effects of drugs.
A: Human INSR Stable Cell Line - HEK293T can provide unique insights into the role of insulin receptor function and insulin signaling in the development of obesity, especially in studying insulin resistance and metabolic disorders associated with obesity. This cell line is a valuable tool for such research.
A: When conducting gene expression analysis, it is important to choose appropriate methods (such as real-time quantitative PCR or RNA sequencing) and strictly control experimental conditions to avoid technical biases. Additionally, the impact of insulin treatment on cellular gene expression and appropriate control experiments should be considered.
A: Human INSR Stable Cell Line - HEK293T can play a role in studying various aspects of metabolic syndrome, including abnormalities in glucose metabolism, insulin resistance, and lipid metabolism disorders. This cell line can be used to evaluate the efficacy of drugs treating these conditions and to study the regulation of metabolic pathways.
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