Human INSR(His) HEK293 Cell Lysate

Name: Human INSR(His) HEK293 Cell Lysate
SKU: CLOE-1006
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1006 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1006
Introduction	This cell lysate over-expresses extracellular domain of human INSR isoform short (NP_001073285.1) (Met 1-Lys 944) fused with a polyhistidine tag at the C-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	INSR
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	INSR insulin receptor [ Homo sapiens ]
Gene Symbol	INSR
Synonyms	HHF5; CD220
Gene Description	insulin receptor
Gene ID	3643
Uni Prot ID	P06213
m RNA Refseq	NM_001079817.1
Protein Refseq	NP_001073285.1
Chromosome Location	19p13.3-p13.2
Function	3-phosphoinositide-dependent protein kinase binding; ATP binding; GTP binding; PTB domain binding; SH2 domain binding; insulin binding; insulin binding; insulin receptor substrate binding; insulin-activated receptor activity; insulin-like growth factor I binding; insulin-like growth factor II binding; insulin-like growth factor receptor binding; lipoic acid binding; phosphatidylinositol 3-kinase binding; protein binding; protein complex binding; protein phosphatase binding; protein tyrosine kinase activity; protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity;
Pathway	AMPK signaling, organism-specific biosystem; Adherens junction, organism-specific biosystem; Adherens junction, conserved biosystem; Aldosterone-regulated sodium reabsorption, organism-specific biosystem; Aldosterone-regulated sodium reabsorption, conserved biosystem; DNA damage response (only ATM dependent), organism-specific biosystem; HIF-1 signaling pathway, organism-specific biosystem;
MIM	147670

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