Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-SC006382-1
Host Cell : CHO-K1 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-SC006382-1 |
| Description | This cell line is engineered to stably express human G protein subunit alpha 15 (GNA15) in CHO-K1 cells. |
| Target Gene | GNA15 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Applications |
1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | GNA15 guanine nucleotide binding protein (G protein), alpha 15 (Gq class) [ Homo sapiens ] |
| Gene Symbol | GNA15 |
| Synonyms | GNA16 |
| Gene Description | guanine nucleotide binding protein (G protein), alpha 15 (Gq class) |
| GeneID | 2769 |
| Uni ProtID | P30679 |
| mRNA Refseq | NM_002068.2 |
| Protein Refseq | NP_002059.2 |
| Chromosome Location | 19p13.3 |
| Function | G-protein beta/gamma-subunit complex binding; G-protein coupled receptor binding; GTP binding; GTPase activity; metal ion binding; signal transducer activity; |
| Pathway | ADP signalling through P2Y purinoceptor 1, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Arf6 signaling events, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem; |
| MIM | 139314 |
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