Human GNA15 Stable Cell Line - CHO-K1

GNA15, officially known as G protein subunit alpha 15, is a human gene that codes for the alpha subunit of the heterotrimeric G protein G15, a member of the Gq class. The protein, also referred to as Gα15 or Gα16, is predominantly expressed in hematopoietic cells and plays a critical role in coupling G protein-coupled receptors (GPCRs) to downstream effectors, most notably phospholipase Cβ, leading to calcium mobilization and activation of protein kinase C. The Human GNA15 Stable Cell Line - CHO-K1 is generated by stably transfecting the full-length human GNA15 cDNA into the Chinese hamster ovary K1 (CHO-K1) host cell line, which does not endogenously express Gα15. This allows for controlled, reproducible expression of the human Gα15 protein in a mammalian cellular environment, providing a clean background for studying receptor-G protein interactions without interference from endogenous signaling components.

This stable cell line is widely used in GPCR deorphanization studies, ligand screening assays, and signal transduction research. Because Gα15 is known to promiscuously couple with a broad range of GPCRs that do not naturally activate the Gq pathway, the cell line serves as a universal tool for converting receptor activation into a measurable calcium response, regardless of the receptor’s native G protein preference. This makes it particularly valuable for high-throughput screening campaigns aimed at identifying novel ligands for orphan receptors or for characterizing the coupling profiles of candidate GPCRs. Researchers also use this line to investigate the mechanistic details of Gα15-mediated signaling, including its interactions with regulators such as RGS proteins and its role in calcium-dependent cellular processes. The stable expression ensures consistent assay performance across experiments, reducing batch-to-batch variability and enabling long-term studies.