Human GNA15 Stable Cell Line-CHO-K1

Name: Human GNA15 Stable Cell Line-CHO-K1
SKU: CSC-RG0846
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0846

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0846
Background	This cell line expresses only the promiscuous G protein Galpha16.
Target Gene	GNA15
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuGNA15
Alias	GNA15, GNA16
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GNA15 guanine nucleotide binding protein (G protein), alpha 15 (Gq class) [ Homo sapiens ]
Gene Symbol	GNA15
Synonyms	GNA16
Gene Description	guanine nucleotide binding protein (G protein), alpha 15 (Gq class)
GeneID	2769
Uni ProtID	P30679
mRNA Refseq	NM_002068.2
Protein Refseq	NP_002059.2
Chromosome Location	19p13.3
Function	G-protein beta/gamma-subunit complex binding; G-protein coupled receptor binding; GTP binding; GTPase activity; metal ion binding; signal transducer activity;
Pathway	ADP signalling through P2Y purinoceptor 1, organism-specific biosystem; Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Arf6 signaling events, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Chagas disease (American trypanosomiasis), organism-specific biosystem;
MIM	139314

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