Cat.No. : CSC-RO0859 Host Cell: CT26
| Cat. No. | CSC-RO0859 |
| Description | This cell line is engineered to stably overexpress human GDF15. |
| Gene | GDF15 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CT26 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human GDF15 Stable Cell Line - CT26 is obtained by introducing the human GDF15 gene into the CT26 cell line, allowing it to stably express the GDF15 protein. This is usually achieved through transfection, viral transduction, or CRISPR/Cas9 gene editing technologies. After successful transfection, the cells with stable GDF15 expression are selected and used for further experimental research.
A: There are several methods to assess the expression level of GDF-15 protein in Human GDF15 Stable Cell Line - CT26, such as enzyme-linked immunosorbent assay (ELISA), protein blotting (Western Blot), and immunofluorescence staining. These methods can help researchers understand the expression levels and subcellular localization of GDF-15 in cells, providing important information for subsequent experiments.
A: The applications of Human GDF15 Stable Cell Line - CT26 in tumor research mainly include: investigating the role of GDF-15 in tumorigenesis, development, and metastasis; evaluating the feasibility of GDF-15 as a potential therapeutic target; studying the interaction between GDF-15 and other signaling pathways; and developing novel anti-tumor drugs targeting GDF-15.
A: When culturing Human GDF15 Stable Cell Line - CT26, factors such as culture conditions, such as temperature, CO2 concentration, and culture medium components, should be carefully controlled. In addition, the medium should be replaced regularly to avoid overcrowding of cells. For cell lines containing antibiotic resistance genes, the appropriate antibiotic should be added to the culture medium.
A: To avoid experimental errors, it is necessary to strictly control experimental conditions, such as the number of cell passages and culture conditions. In addition, the experimental design should include appropriate control groups, such as cells transfected with empty vectors or untreated cells. At the same time, the experimental results should be verified through multiple repetitions of the experiments.
A: The Human GDF15 Stable Cell Line - CT26 can be used for drug screening, especially for drugs targeting the GDF15 pathway. By adding different drugs to the cells and observing their effects on GDF15 expression and cell function, potential drug candidates can be screened.
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