Human GDF15 Stable Cell Line - CT26

Name: Human GDF15 Stable Cell Line - CT26
SKU: CSC-RO0859
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0859

Host Cell : CT26 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0859
Description	This cell line is engineered to stably overexpress human GDF15.
Target Gene	GDF15
Gene Species	Homo sapiens (Human)
Host Cell	CT26
Host Cell Species	Mus musculus (Mouse)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GDF15 growth differentiation factor 15 [ Homo sapiens ]
Gene Symbol	GDF15
Synonyms	PDF; MIC1; PLAB; MIC-1; NAG-1; PTGFB; GDF-15
Gene Description	growth differentiation factor 15
GeneID	9518
Uni ProtID	Q99988
mRNA Refseq	NM_004864.2
Protein Refseq	NP_004855.2
Chromosome Location	19p13.11
Function	cytokine activity; growth factor activity;
Pathway	Direct p53 effectors, organism-specific biosystem;
MIM	605312

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How is the Human GDF15 Stable Cell Line - CT26 obtained through genetic engineering methods?

A: The Human GDF15 Stable Cell Line - CT26 is obtained by introducing the human GDF15 gene into the CT26 cell line, allowing it to stably express the GDF15 protein. This is usually achieved through transfection, viral transduction, or CRISPR/Cas9 gene editing technologies. After successful transfection, the cells with stable GDF15 expression are selected and used for further experimental research.

How to evaluate the expression level of GDF-15 protein in Human GDF15 Stable Cell Line - CT26 during research?

A: There are several methods to assess the expression level of GDF-15 protein in Human GDF15 Stable Cell Line - CT26, such as enzyme-linked immunosorbent assay (ELISA), protein blotting (Western Blot), and immunofluorescence staining. These methods can help researchers understand the expression levels and subcellular localization of GDF-15 in cells, providing important information for subsequent experiments.

What are the applications of Human GDF15 Stable Cell Line - CT26 in tumor research?

A: The applications of Human GDF15 Stable Cell Line - CT26 in tumor research mainly include: investigating the role of GDF-15 in tumorigenesis, development, and metastasis; evaluating the feasibility of GDF-15 as a potential therapeutic target; studying the interaction between GDF-15 and other signaling pathways; and developing novel anti-tumor drugs targeting GDF-15.

What factors should be considered when culturing Human GDF15 Stable Cell Line - CT26 to maintain its stability?

A: When culturing Human GDF15 Stable Cell Line - CT26, factors such as culture conditions, such as temperature, CO2 concentration, and culture medium components, should be carefully controlled. In addition, the medium should be replaced regularly to avoid overcrowding of cells. For cell lines containing antibiotic resistance genes, the appropriate antibiotic should be added to the culture medium.

How can experimental errors be avoided when using Human GDF15 Stable Cell Line - CT26 for experiments?

A: To avoid experimental errors, it is necessary to strictly control experimental conditions, such as the number of cell passages and culture conditions. In addition, the experimental design should include appropriate control groups, such as cells transfected with empty vectors or untreated cells. At the same time, the experimental results should be verified through multiple repetitions of the experiments.

What is the application of Human GDF15 Stable Cell Line - CT26 in drug screening?

A: The Human GDF15 Stable Cell Line - CT26 can be used for drug screening, especially for drugs targeting the GDF15 pathway. By adding different drugs to the cells and observing their effects on GDF15 expression and cell function, potential drug candidates can be screened.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *