Cat.No. : CSC-RO0899 Host Cell: NCI-H146
| Cat. No. | CSC-RO0899 |
| Description | This cell line is engineered to stably overexpress human CD274 (also named as PDL1). |
| Gene | CD274 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | NCI-H146 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CD274 Stable Cell Line - NCI-H146 is a stable cell line derived from the human lung cancer cell line NCI-H146. This cell line has been stably transfected or engineered to express the CD274 gene, also known as PD-L1 (Programmed Death-Ligand 1). PD-L1 is an immune checkpoint molecule associated with immune evasion in cancer. This cell line is used for research in immunotherapy, immune checkpoint inhibitors, and cancer immune evasion mechanisms.
A: NCI-H146 was chosen as the source for the Human CD274 Stable Cell Line because it is a human lung cancer cell line commonly used in lung cancer and immunotherapy research. PD-L1 (CD274) expression in lung cancer is closely associated with immune evasion, making this cell line valuable for studying the biological functions of PD-L1 and drug screening for PD-L1 inhibitors.
A: PD-L1 (CD274) gene expression in the Human CD274 Stable Cell Line - NCI-H146 is typically achieved through stable gene expression or transfection. These cells are maintained under appropriate culture conditions, which often involve the addition of suitable culture media and antibiotics to sustain gene expression. Regular passaging and monitoring of the cells are crucial for maintaining stability.
A: The Human CD274 Stable Cell Line - NCI-H146 has broad potential in immunotherapy research. It can be used to assess the effectiveness of PD-L1 inhibitors, study the mechanisms of immune checkpoint inhibitors, and develop new immunotherapy strategies, including CAR-T cell therapy and cancer vaccines.
A: PD-L1 (CD274) is an important target in immune checkpoint inhibitor therapy. It inhibits T cell activation by interacting with the PD-1 receptor, thereby helping cancer cells evade immune attack. Inhibiting the interaction between PD-L1 and PD-1 can restore T cell activation, enhance immune responses, and improve resistance against tumors.
A: The Human CD274 Stable Cell Line - NCI-H146 can be used to study various types of immune checkpoint inhibitors, including anti-PD-L1 antibodies, anti-PD-1 antibodies, and other related immunotherapy drugs. This helps evaluate their efficacy and mechanisms.
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