Human CD274 Stable Cell Line - NCI-H146

Name: Human CD274 Stable Cell Line - NCI-H146
SKU: CSC-RO0899
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0899

Host Cell : NCI-H146 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0899
Description	This cell line is engineered to stably overexpress human CD274 (also named as PDL1).
Target Gene	CD274
Gene Species	Homo sapiens (Human)
Host Cell	NCI-H146
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD274 CD274 molecule [ Homo sapiens ]
Gene Symbol	CD274
Synonyms	CD274; CD274 molecule; CD274 antigen , PDCD1LG1, programmed cell death 1 ligand 1; programmed cell death 1 ligand 1; B7 homolog 1; B7 H; B7 H1; B7H1; PD L1; PDL1; CD274 antigen; PDCD1 ligand 1; programmed death ligand 1; B7-H; PD-L1; PDCD1L1; PDCD1LG1; MGC142294; MGC142296;
Gene ID	29126
Uni Prot ID	Q9NZQ7
m RNA Refseq	BC113734
Chromosome Location	9p24.1
Function	protein binding; protein tyrosine phosphatase activity; receptor activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; PD-1 signaling, organism-specific biosystem;
MIM	605402

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Customer Reviews

Customer Q&As

What type of cell line is the Human CD274 Stable Cell Line - NCI-H146, and what are its characteristics and applications?

A: The Human CD274 Stable Cell Line - NCI-H146 is a stable cell line derived from the human lung cancer cell line NCI-H146. This cell line has been stably transfected or engineered to express the CD274 gene, also known as PD-L1 (Programmed Death-Ligand 1). PD-L1 is an immune checkpoint molecule associated with immune evasion in cancer. This cell line is used for research in immunotherapy, immune checkpoint inhibitors, and cancer immune evasion mechanisms.

Why was NCI-H146 chosen as the source for this cell line?

A: NCI-H146 was chosen as the source for the Human CD274 Stable Cell Line because it is a human lung cancer cell line commonly used in lung cancer and immunotherapy research. PD-L1 (CD274) expression in lung cancer is closely associated with immune evasion, making this cell line valuable for studying the biological functions of PD-L1 and drug screening for PD-L1 inhibitors.

How is the PD-L1 (CD274) gene expression in Human CD274 Stable Cell Line - NCI-H146 maintained?

A: PD-L1 (CD274) gene expression in the Human CD274 Stable Cell Line - NCI-H146 is typically achieved through stable gene expression or transfection. These cells are maintained under appropriate culture conditions, which often involve the addition of suitable culture media and antibiotics to sustain gene expression. Regular passaging and monitoring of the cells are crucial for maintaining stability.

What is the potential of the Human CD274 Stable Cell Line - NCI-H146 in immunotherapy research?

A: The Human CD274 Stable Cell Line - NCI-H146 has broad potential in immunotherapy research. It can be used to assess the effectiveness of PD-L1 inhibitors, study the mechanisms of immune checkpoint inhibitors, and develop new immunotherapy strategies, including CAR-T cell therapy and cancer vaccines.

What is the mechanism of action of PD-L1 (CD274) in immune checkpoint inhibitor therapy?

A: PD-L1 (CD274) is an important target in immune checkpoint inhibitor therapy. It inhibits T cell activation by interacting with the PD-1 receptor, thereby helping cancer cells evade immune attack. Inhibiting the interaction between PD-L1 and PD-1 can restore T cell activation, enhance immune responses, and improve resistance against tumors.

What types of immune checkpoint inhibitors can be studied using the Human CD274 Stable Cell Line - NCI-H146?

A: The Human CD274 Stable Cell Line - NCI-H146 can be used to study various types of immune checkpoint inhibitors, including anti-PD-L1 antibodies, anti-PD-1 antibodies, and other related immunotherapy drugs. This helps evaluate their efficacy and mechanisms.

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