Caspase-8 Activity Assay Kit

Name: Caspase-8 Activity Assay Kit
SKU: PTAK-009

For research use only. Not intended for any clinical use.

Cat.No.

PTAK-009

Description

The Caspase-8 Assay Kit is a complete assay system designed to measure Caspase-8 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-8 activity measurements. In addition, the kit includes purified Caspase-8 enzyme and a potent Caspase-8 inhibitor, Ac-IETD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-8 activity level. The fluorogenic substrate, Ac-IETD-AFC, is incubated with purified Caspase-8 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.

Applications

Great for studying enzyme kinetics and HTS applications.

Caution

Avoid freeze/thaw cycles

Component

•Caspase-8
•Caspase-8 assay buffer
•Caspase-8 substrate
•DTT
•Caspase-8 inhibitor (Ac-IETD-SHO)
•Microtiter plate

Method

Fluorescence

Size/Form

100 assays

Publications

Q & A

Customer Reviews

Customer Q&As

What is caspase-8?

A: Caspase-8 is also known as "apoptotic protease activating factor 1" (APAF1). It is an enzyme that plays a key role in initiating apoptosis, which is a process of programmed cell death.

What is the role of caspase-8 in apoptosis?

A: Caspase-8 is involved in initiating the extrinsic pathway of apoptosis, which is triggered by external signals such as cell surface death receptors.

What diseases are caspase-8 associated with?

A: Caspase-8 is essential for maintaining normal cellular homeostasis and development, and its dysregulation has been associated with various diseases, including cancer, autoimmune disorders, and neurodegenerative diseases.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

First Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *