Cathepsin B Activity Assay Kit

Name: Cathepsin B Activity Assay Kit
SKU: PTAK-012

For research use only. Not intended for any clinical use.

Cat.No.

PTAK-012

Description

The Cathepsin B Activity Assay Kit is designed to measure the protease activity of Cathepsin B for screening and profiling applications. The Cathepsin B assay kit comes in a convenient 96-well format, with purified Cathepsin B, its fluorogenic substrate, and Cathepsin buffer for 100 enzyme reactions. In addition, the kit includes the cathepsin inhibitor E64 for use as a control inhibitor.

Applications

Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.

Caution

Avoid freeze/thaw cycles

Component

•Cathepsin B
•Fluorogenic Cathepsin substrate
•assay buffer
•Cathepsin B inhibitor E64
•96-well microtiter plate

Method

Fluorescence

Size/Form

100 assays

Publications

Q & A

Customer Reviews

Customer Q&As

What is Cathepsin B?

A: Cathepsin B belongs to a family of lysosomal cysteine proteases called cysteine cathepsins that play an important role in intracellular proteolysis.

What is the role of cathepsin B in physiological processes?

A: Cathepsin B plays a crucial role in various physiological processes, including protein degradation and turnover, antigen processing, apoptosis, and tissue remodeling.

How is cathepsin B synthesized?

A: Cathepsin B is synthesized on the rough endoplasmic reticulum as a preproenzyme of 339 amino acids with a signal peptide of 17 amino acids. Procathepsin B of 43/46 kDa is then transported to the Golgi apparatus, where cathepsin B is formed.

What is the structure of cathepsin B?

A: Mature cathepsin B is composed of a heavy chain of 25-26 kDa and a light chain of 5kDa, which are linked by a dimer of disulfide.

What diseases are cathepsin B associated with?

A: In pathological situations, cathepsin B can contribute to various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases.

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