Plasma Kallikrein Activity Assay Kit

Name: Plasma Kallikrein Activity Assay Kit
SKU: PTAK-034

For research use only. Not intended for any clinical use.

Cat.No.

PTAK-034

Description

The Plasma Kallikrein Activity Assay Kit utilizes the ability of active Plasma Kallikrein to cleave a synthetic pNA-based peptide substrate to release pNA (OD405 nm), which can be easily quantified using a microplate reader. The Plasma Kallikrein Specific Inhibitor (PKSI) selectively inhibits the ability of Plasma Kallikrein to the cleave the synthetic substrate. The kit is easy-to-use and can detect PK activity of Purified Plasma Kallikrein and Plasma Samples

Applications

Useful for detecting the activity of plasma kallikrein in plasma samples and purified/crude enzyme preparations.

Caution

Avoid freeze/thaw cycles

Component

•Active PK enzyme
•PK assay buffer
•PK activator
•PK inhibitor
•pNA standard

Method

Colorimetric

Size/Form

100 assays

Publications

Q & A

Customer Reviews

Customer Q&As

What is plasma kallikrein?

A: Plasma kallikrein is an enzyme found in the blood plasma that plays a role in the clotting and inflammatory responses of the body.

What is the function of plasma kallikrein?

A: Plasma kallikrein is a protease enzyme that cleaves specific proteins called kininogens, leading to the production of bradykinin, a potent vasodilator and mediator of inflammation.

What is the structure of plasma kallikrein?

A: Plasma kallikrein consists of heavy and light chains linked together by disulfide bonds. The heavy chain is derived from the amino terminus of the zymogen and consists of four tandem repeats of 90 or 91 amino acids. Each repeat contains a novel structure called an apple domain. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases.

What diseases are plasma kallikrein associated with?

A: Dysregulation of plasma kallikrein activity is associated with various diseases, including hereditary angioedema, inflammatory conditions, and cardiovascular disorders.

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