Alkaline Phosphatase Assay Kit

Name: Alkaline Phosphatase Assay Kit
SKU: PAK-003
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : PAK-003

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Product Information

Cat. No.	PAK-003
Description	The Alkaline Phosphatase Assay Kit is a highly sensitive kit to detect alkaline phosphatase (ALP) activity in serum and other biological samples. In the assay, ALP dephosphorylates the pNPP (p-Nitrophenyl phosphate) phosphatase substrate and releases pNPP, resulting in a colorimetic detectable signal at absorbance=405 nm. By employing an ALP activity standard curve, the active ALP in test samples can be measured within 1 hour in a sensitive manner.
Component	Lysis Buffer ALP Assay Buffer ALP Substrate Stop Solution ALP standard
Applications	Useful for detecting ALPs and their corresponding modulators and inhibitors.
Size	500 Assays
Method	Colorimetric
Caution	Avoid freeze/thaw cycles

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What is alkaline phosphatase?

A: Alkaline phosphatase is an enzyme that catalyzes the hydrolysis of phosphate groups from organic phosphomonoesters in alkaline conditions.

Where is alkaline phosphatase primarily expressed?

A: It is found in various tissues and organs throughout the body, including the liver, bones, kidneys, and intestines.

What is the alkaline phosphatase?

A: Alkaline phosphatase is a homodimeric enzyme, which means it is formed from two molecules. The catalytic site contains three metal ions, two zinc ions and one magnesium ion, both types of which are essential for the enzyme activity to occur.

What is the role of alkaline phosphatasein in physiological processes?

A: Alkaline phosphatase plays a crucial role in various physiological processes, such as bone formation and mineralization, liver function, and cellular signaling.

What are the research applications of alkaline phosphatasein in E. coli?

A: By changing the amino acids of wild-type alkaline phosphatase produced by E. coli, a mutant alkaline phosphatase was produced that not only increased the enzyme activity 36-fold but also retained thermal stability.

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