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PAK-005

Calcineurin Phosphatase Activity Assay Kit

For research use only. Not intended for any clinical use.

Cat. No. :   PAK-005

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Cat. No. PAK-005
Description The Calcineurin Phosphatase Activity Assay Kit is a complete non-radioactive colorimetric assay kit designed for measuring calcineurin phosphatase activity through kinetic assay or for inhibitor screening. The activity is determined using the RII phosphopeptide substrate, the most efficient and selective peptide known for calcineurin, and detection of free phosphate released is based on the classic malachite green assay.
Component •Active calcineurin enzyme
•calcineurin assay buffer
•Calcineurin substrate
•Calmodulin
•Phosphate standard
•96-well microplate
Applications Useful for detecting the activity of calcineurin phosphatase
Size 100 assays
Method Colorimetric
Caution Avoid freeze/thaw cycles
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What is calcineurin phosphatase?

A: Calcineurin phosphatase, also known as protein phosphatase 3 (PP3), is an important enzyme that plays a critical role in signal transduction pathways in cells.

What is the structure of calcineurin phosphatase?

A: Calcineurin phosphatase is composed of three subunits: a catalytic subunit (calcineurin A), a regulatory subunit (calcineurin B), and a calcium-binding protein (calmodulin).

What is the role of calcineurin phosphatase in cellular processes?

A: Calcineurin phosphatase is a calcium/calmodulin-dependent serine/threonine protein phosphatase and is involved in numerous cellular processes such as neuronal development, immune responses, muscle activity, and cardiac function.

What diseases are calcineurin associated with?

A: In addition to its role in immune and cardiac diseases, calcineurin has been implicated in neurodegenerative diseases such as Alzheimer's disease and neurodevelopmental disorders such as autism and schizophrenia.

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