Caspase-3 Activity Assay Kit

Name: Caspase-3 Activity Assay Kit
SKU: PTAK-004

For research use only. Not intended for any clinical use.

Cat.No.

PTAK-004

Description

The caspase-3 Activity Assay Kit comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-3 activity measurements. In addition, the kit includes purified Caspase-3 enzyme and a potent Caspase-3 inhibitor, Ac-DNLD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-3 activity level. The fluorogenic substrate, Ac-DEVD-AFC, is incubated with purified Caspase-3 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.

Applications

Useful for studying enzyme kinetics and HTS applications.

Caution

Avoid freeze/thaw cycles

Component

•Caspase-3
•Caspase-3 assay buffer
•Caspase-3 substrate
•DTT
•Caspase-3 inhibitor (Ac-DNLD-CHO)
•Microtiter plate

Method

Fluorescence

Size/Form

100 assays

Publications

Q & A

Customer Reviews

Customer Q&As

What is Caspase-3？

A: Caspase-3 is a member of the caspase family of proteins, which are involved in various stages of apoptosis.

What is the function of caspase-3?

A: Caspase-3 is a crucial enzyme involved in apoptosis, also known as programmed cell death. It is responsible for the execution phase of apoptosis, where it cleaves various proteins and ultimately leads to the destruction of the cell.

How is caspase-3 activated?

A: Caspase-3 activation occurs through a cascade of proteolytic cleavage events, which are triggered by various upstream signaling pathways. In response to apoptotic signals, initiator caspases, such as caspase-8 or caspase-9, become activated. These initiator caspases then cleave and activate downstream effector caspases, including caspase-3.

What is the role of caspase-3 in neurodegenerative diseases?

A: In diseases like Alzheimer's, Parkinson's, and Huntington's disease, caspase-3 activation plays a role in neuronal apoptosis, contributing to the progressive loss of brain cells.

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