Rat Drd4 Stable Cell Line-CHO-K1

Name: Rat Drd4 Stable Cell Line-CHO-K1
SKU: CSC-RG1154
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG1154

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG1154
Description	This cell line is engineered to stably overexpress Rat Drd4 in CHO-K1 cells.
Target Gene	Drd4
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Drd4 dopamine receptor D4 [ Mus musculus ]
Gene Symbol	Drd4
Synonyms	D4R; Drd-4; AW125663
Gene Description	dopamine receptor 4
Gene ID	13491
Uni Prot ID	P51436
m RNA Refseq	NM_007878.2
Protein Refseq	NP_031904.1
Chromosome Location	7 F5; 7 70.1 cM
Function	G-protein coupled receptor activity; SH3 domain binding; dopamine binding; dopamine receptor activity; dopamine receptor activity, coupled via Gi/Go; dopamine receptor activity, coupled via Gi/Go; drug binding; signal transducer activity;
Pathway	Amine ligand-binding receptors, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Dopamine receptors, organism-specific biosystem; Dopaminergic synapse, organism-specific biosystem; Dopaminergic synapse, conserved biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem;

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