Mouse Tnfrsf14 Stable Cell Line - CHO-K1

Name: Mouse Tnfrsf14 Stable Cell Line - CHO-K1
SKU: CSC-RO0206
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0206

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0206
Description	This cell line is engineered to stably overexpress the mouse Tnfrsf14 in CHO-K1 cells.
Introduction	This cell line is constructed by transfection of mouse tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) (Tnfrsf14) into CHO-K1, followed by stable cell selection. The expression of Tnfrsf14 has been analyzed through flow cytometry.
Target Gene	Tnfrsf14
Gene Species	Mus musculus (Mouse)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Tnfrsf14 tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) [ Mus musculus ]
Gene Symbol	Tnfrsf14
Synonyms	Atar; HveA; Hvem; Tnfrs14
Gene Description	tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
Gene ID	230979
Uni Prot ID	Q80WM9
m RNA Refseq	NM_178931.2
Protein Refseq	NP_849262.1
Chromosome Location	4 E2; 4
Function	protein binding; ubiquitin protein ligase binding;
Pathway	Adaptive Immune System, organism-specific biosystem; Costimulation by the CD28 family, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; Immune System, organism-specific biosystem;

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Q & A

Customer Reviews

Customer Q&As

What is the origin of the Mouse Tnfrsf14 Stable Cell Line - CHO-K1?

A: The Mouse Tnfrsf14 Stable Cell Line - CHO-K1 is derived from Chinese Hamster Ovary (CHO-K1) cells, which are a commonly used cell line in biotechnology and research.

What is the specific genotype and phenotype of the Mouse Tnfrsf14 Stable Cell Line - CHO-K1?

A: The specific genotype and phenotype of the Mouse Tnfrsf14 Stable Cell Line - CHO-K1 would depend on the genetic modifications introduced to express the Tnfrsf14 gene. CHO-K1 cells are known for their ability to grow in suspension culture and are often used for protein expression.

What are the recommended storage and revival procedures for the Mouse Tnfrsf14 Stable Cell Line - CHO-K1?

A: The storage and revival procedures for the Mouse Tnfrsf14 Stable Cell Line - CHO-K1 may involve freezing the cells in a cryoprotective medium at a specific temperature and then thawing and culturing them as needed. The exact protocols should be provided by the cell line provider or developed based on best practices for CHO-K1 cells.

What are the biosafety precautions that researchers should take when working with the Mouse Tnfrsf14 Stable Cell Line - CHO-K1?

A: Researchers working with the Mouse Tnfrsf14 Stable Cell Line - CHO-K1 should follow standard laboratory biosafety practices, including using appropriate personal protective equipment, working in a certified biosafety cabinet, and handling potentially hazardous materials in accordance with established protocols. Specific precautions may also depend on the properties of the modified cell line.

Are there any unique considerations or challenges associated with using the Mouse Tnfrsf14 Stable Cell Line - CHO-K1 in research compared to other cell lines?

A: The specific considerations and challenges when working with the Mouse Tnfrsf14 Stable Cell Line - CHO-K1 may include optimizing culture conditions for protein production, studying the signaling pathways involving Tnfrsf14, and ensuring the stability of the introduced genetic modifications over time.

What are the primary applications of the Mouse Tnfrsf14 Stable Cell Line - CHO-K1 in research or biotechnology?

A: The Mouse Tnfrsf14 Stable Cell Line - CHO-K1 can be used for various applications, including the production of recombinant proteins, studying Tnfrsf14 (tumor necrosis factor receptor superfamily 14) biology, and investigating signaling pathways associated with this receptor.

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