Cat.No. : CSC-RO0206 Host Cell: CHO-K1
| Cat. No. | CSC-RO0206 |
| Description | This cell line is engineered to stably overexpress the mouse Tnfrsf14 in CHO-K1 cells. |
| Introduction | This cell line is constructed by transfection of mouse tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) (Tnfrsf14) into CHO-K1, followed by stable cell selection. The expression of Tnfrsf14 has been analyzed through flow cytometry. |
| Gene | Tnfrsf14 |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Mouse Tnfrsf14 Stable Cell Line - CHO-K1 is derived from Chinese Hamster Ovary (CHO-K1) cells, which are a commonly used cell line in biotechnology and research.
A: The specific genotype and phenotype of the Mouse Tnfrsf14 Stable Cell Line - CHO-K1 would depend on the genetic modifications introduced to express the Tnfrsf14 gene. CHO-K1 cells are known for their ability to grow in suspension culture and are often used for protein expression.
A: The storage and revival procedures for the Mouse Tnfrsf14 Stable Cell Line - CHO-K1 may involve freezing the cells in a cryoprotective medium at a specific temperature and then thawing and culturing them as needed. The exact protocols should be provided by the cell line provider or developed based on best practices for CHO-K1 cells.
A: Researchers working with the Mouse Tnfrsf14 Stable Cell Line - CHO-K1 should follow standard laboratory biosafety practices, including using appropriate personal protective equipment, working in a certified biosafety cabinet, and handling potentially hazardous materials in accordance with established protocols. Specific precautions may also depend on the properties of the modified cell line.
A: The specific considerations and challenges when working with the Mouse Tnfrsf14 Stable Cell Line - CHO-K1 may include optimizing culture conditions for protein production, studying the signaling pathways involving Tnfrsf14, and ensuring the stability of the introduced genetic modifications over time.
A: The Mouse Tnfrsf14 Stable Cell Line - CHO-K1 can be used for various applications, including the production of recombinant proteins, studying Tnfrsf14 (tumor necrosis factor receptor superfamily 14) biology, and investigating signaling pathways associated with this receptor.
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