Human TNFRSF14 Stable Cell Line - CHO-K1

Name: Human TNFRSF14 Stable Cell Line - CHO-K1
SKU: CSC-RO0205
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0205

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0205
Description	This cell line is engineered to stably overexpress the human TNFRSF14 in CHO-K1 cells.
Introduction	This cell line is constructed by transfection of human TNF receptor superfamily member 14 (TNFRSF14) into CHO-K1, followed by stable cell selection. The expression of TNFRSF14 has been analyzed through flow cytometry.
Target Gene	TNFRSF14
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFRSF14 tumor necrosis factor receptor superfamily, member 14 [ Homo sapiens ]
Gene Symbol	TNFRSF14
Synonyms	TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
Gene Description	tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator)
Gene ID	8764
Uni Prot ID	Q92956
m RNA Refseq	NM_003820.2
Protein Refseq	NP_003811.2
Chromosome Location	1p36.32
Function	protein binding; tumor necrosis factor-activated receptor activity; ubiquitin protein ligase binding;
Pathway	Adaptive Immune System, organism-specific biosystem; Costimulation by the CD28 family, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem; Immune System, organism-specific biosystem;
MIM	602746

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