Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RO01369
Host Cell : Jurkat, Clone E6-1 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RO01369 |
| Description | This cell line is engineered to stably express Mus musculus (Murine, House mouse) tumor necrosis factor receptor superfamily, member 9 (Tnfrsf9) in Human immortalized T lymphoblastoid cell line (Jurkat, Clone E6-1). GFP reporter gene is also expressed in this cell line allowing fluorescent tracking of cells. |
| Product Type | Mouse gene overexpression stable cell line |
| Target Gene | Tnfrsf9 |
| Gene Species | Mus musculus (Murine, House mouse) |
| Host Cell | Jurkat, Clone E6-1 |
| Host Cell Species | Homo sapiens (Human) |
| Reporter | GFP |
| Applications |
1) investigation of gene function 2) screening and validation of antibodies |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) Real-time qPCR analysis of gene mRNA overexpression level 2) GFP fluorescent detection under fluorescent microscopy 3) mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Suspension |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | TNFRSF9 |
| Background | The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. [provided by RefSeq, Jul 2008] |
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We are very pleased with the Mouse Tnfrsf9 (4-1BB) Jurkat cell line. The responsiveness of the line in our activation assays was exactly as described. Creative Biogene’s quality control is evident, as the cells arrived in great condition and were easy to expand.
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