Human P2RX7 Stable Cell Line-HEK293

Name: Human P2RX7 Stable Cell Line-HEK293
SKU: CSC-RI0190
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0190

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0190
Description	This cell line is engineered to overexpress human P2RX7.
Background	The human P2RX7 gene encodes P2X7, an ionotropic ATP receptor that functions as an ATP-activated cation channel expressed in lymphocytes and microglia. P2X7 receptors are therapeutic targets in treatment of osteoporosis, neuropathic and inflammatory pain, and immune diseases.
Target Gene	P2RX7
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuP2RX7
Alias	P2RX7, MGC20089, P2X7
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	P2RX7 purinergic receptor P2X, ligand-gated ion channel, 7 [ Homo sapiens ]
Gene Symbol	P2RX7
Synonyms	P2RX7; purinergic receptor P2X, ligand-gated ion channel, 7; P2X purinoceptor 7; MGC20089; P2X7; ATP receptor; P2Z receptor; P2X7 receptor; purinergic receptor P2X7 variant A;
Gene ID	5027
Uni Prot ID	Q99572
m RNA Refseq	BC011913
Chromosome Location	12q24
Function	ATP binding; ATP binding; extracellular ATP-gated cation channel activity; ion channel activity; lipopolysaccharide binding; protein homodimerization activity; purinergic nucleotide receptor activity; receptor activity; receptor binding;
Pathway	Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Immune System, organism-specific biosystem; Inflammasomes, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem;
MIM	602566

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Q & A

Customer Reviews

Customer Q&As

How was the Human P2RX7 Stable Cell Line-HEK293 established?

A: The cell line was established by stably transfecting the human P2RX7 (P2X purinoceptor 7) gene into HEK293 cells.

What is the application of the Human P2RX7 Stable Cell Line-HEK293 in studying inflammation and immune responses?

A: This cell line can be used to study the role of P2RX7 in inflammatory responses and the activation of immune cells.

What are the advantages of using the Human P2RX7 Stable Cell Line-HEK293 for drug screening?

A: It can be used for screening drugs that affect P2RX7 activity, especially in the study of anti-inflammatory and immunomodulatory drugs.

What is the application of this cell line in studying cell apoptosis and cell survival mechanisms?

A: Since P2RX7 is closely related to cell apoptosis and survival, this cell line can be used to study the molecular mechanisms of these processes.

What are the potential uses of the Human P2RX7 Stable Cell Line-HEK293 in researching neurodegenerative diseases?

A: The P2RX7 receptor is closely associated with neuroinflammatory processes. This cell line can be used to study the role of P2RX7 in neurodegenerative diseases like Alzheimer's and Parkinson's disease, particularly in exploring disease mechanisms and potential drug targets.

What specific advantages does the Human P2RX7 Stable Cell Line-HEK293 offer in cell signaling research?

A: Since P2RX7 is a crucial extracellular ATP receptor, this cell line is particularly suitable for studying how extracellular ATP signaling affects cell functions, including changes in intracellular calcium concentrations, cell apoptosis, and the regulation of inflammatory responses.

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