Cat.No. : CSC-RG0050 Host Cell: HEK293
| Cat. No. | CSC-RG0050 |
| Background | The protein encoded by this gene is a membrane-bound receptor and member of the melanocortin receptor family. The encoded protein interacts with adrenocorticotropic and MSH hormones and is mediated by G proteins. This is an intronless gene. Defects in this gene are a cause of autosomal dominant obesity. |
| Gene | MC4R |
| Gene Species | Homo sapiens (Human) |
| Alias | MC4R, MC4-R |
| Host Cell | HEK293 |
| Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human MC4R-SNAP Stable Cell Line-HEK293 can be used to study the effects of various drugs on the melanocortin 4 receptor (MC4R), which plays a crucial role in energy homeostasis and appetite regulation. This cell line can be employed in high-throughput screening assays to identify compounds that modulate MC4R activity, potentially leading to the development of new treatments for obesity and related metabolic disorders.
A: The SNAP tag (Single Amino Acid Polymorphism) allows for the specific labeling of the MC4R receptor with biotin. This enables researchers to track the receptor's movement within the cell, study its internalization and recycling, and investigate the impact of various factors on these processes. The SNAP tag's specificity and the ability to label the receptor without affecting its function make it a valuable tool for studying receptor dynamics in the HEK293 cell line.
A: The Human MC4R-SNAP Stable Cell Line-HEK293 provides a platform for investigating the downstream signaling cascade initiated by MC4R activation. Researchers can use this cell line to study the activation of intracellular signaling molecules, such as cAMP, MAPK, and PI3K/Akt pathways, and their roles in regulating cellular processes like energy balance and food intake.
A: Yes, the Human MC4R-SNAP Stable Cell Line-HEK293 can be genetically engineered to express different MC4R variants found in human populations. This allows researchers to study the functional consequences of these genetic variations, such as their impact on receptor activity, ligand binding, and signaling, which can contribute to the understanding of the molecular basis of diseases like obesity and type 2 diabetes.
A: When using the Human MC4R-SNAP Stable Cell Line-HEK293 in drug discovery, researchers must consider the cell line's specificity, sensitivity, and reproducibility. Additionally, it's essential to validate the results in other cell types and animal models to ensure the therapeutic compounds' efficacy and safety before moving to clinical trials.
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