Human MC4R-SNAP Stable Cell Line-HEK293

Name: Human MC4R-SNAP Stable Cell Line-HEK293
SKU: CSC-RG0050
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0050

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0050
Background	The protein encoded by this gene is a membrane-bound receptor and member of the melanocortin receptor family. The encoded protein interacts with adrenocorticotropic and MSH hormones and is mediated by G proteins. This is an intronless gene. Defects in this gene are a cause of autosomal dominant obesity.
Target Gene	MC4R
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuMC4R-SNAP
Alias	MC4R, MC4-R
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	MC4R melanocortin 4 receptor [ Homo sapiens ]
Gene Symbol	MC4R
Gene Description	melanocortin 4 receptor
Gene ID	4160
Uni Prot ID	P32245
m RNA Refseq	NM_005912.2
Protein Refseq	NP_005903.2
Chromosome Location	18q22
Function	melanocortin receptor activity; melanocyte-stimulating hormone receptor activity; neuropeptide binding; peptide hormone binding; protein binding; ubiquitin protein ligase binding;
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (s) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Neuroactive ligand-receptor interaction, organism-specific biosystem; Neuroactive ligand-receptor interaction, conserved biosystem;
MIM	155541

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What are the potential applications of the Human MC4R-SNAP Stable Cell Line-HEK293 in the field of pharmacology?

A: The Human MC4R-SNAP Stable Cell Line-HEK293 can be used to study the effects of various drugs on the melanocortin 4 receptor (MC4R), which plays a crucial role in energy homeostasis and appetite regulation. This cell line can be employed in high-throughput screening assays to identify compounds that modulate MC4R activity, potentially leading to the development of new treatments for obesity and related metabolic disorders.

How does the SNAP tag in the Human MC4R-SNAP Stable Cell Line-HEK293 facilitate the study of receptor trafficking and dynamics?

A: The SNAP tag (Single Amino Acid Polymorphism) allows for the specific labeling of the MC4R receptor with biotin. This enables researchers to track the receptor's movement within the cell, study its internalization and recycling, and investigate the impact of various factors on these processes. The SNAP tag's specificity and the ability to label the receptor without affecting its function make it a valuable tool for studying receptor dynamics in the HEK293 cell line.

How does the Human MC4R-SNAP Stable Cell Line-HEK293 contribute to the understanding of MC4R signaling pathways?

A: The Human MC4R-SNAP Stable Cell Line-HEK293 provides a platform for investigating the downstream signaling cascade initiated by MC4R activation. Researchers can use this cell line to study the activation of intracellular signaling molecules, such as cAMP, MAPK, and PI3K/Akt pathways, and their roles in regulating cellular processes like energy balance and food intake.

Can the Human MC4R-SNAP Stable Cell Line-HEK293 be used to model genetic variations of MC4R associated with human diseases?

A: Yes, the Human MC4R-SNAP Stable Cell Line-HEK293 can be genetically engineered to express different MC4R variants found in human populations. This allows researchers to study the functional consequences of these genetic variations, such as their impact on receptor activity, ligand binding, and signaling, which can contribute to the understanding of the molecular basis of diseases like obesity and type 2 diabetes.

What are the considerations for using the Human MC4R-SNAP Stable Cell Line-HEK293 in drug discovery for MC4R-targeted therapies?

A: When using the Human MC4R-SNAP Stable Cell Line-HEK293 in drug discovery, researchers must consider the cell line's specificity, sensitivity, and reproducibility. Additionally, it's essential to validate the results in other cell types and animal models to ensure the therapeutic compounds' efficacy and safety before moving to clinical trials.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *