Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RG1681
Host Cell : CHO-K1 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RG1681 |
| Background | The protein encoded by this gene is a galanin-like G protein-coupled receptor that binds metastin, a peptide encoded by the metastasis suppressor gene KISS1. The tissue distribution of the expressed gene suggests that it is involved in the regulation of endocrine function, and this is supported by the finding that this gene appears to play a role in the onset of puberty. Mutations in this gene have been associated with hypogonadotropic hypogonadism and central precocious puberty. |
| Target Gene | KISS1R |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Source | CHO-K1 |
| Applications |
1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry Ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | KISS1R KISS1 receptor [ Homo sapiens ] |
| Gene Symbol | KISS1R |
| Synonyms | KISS1R; AXOR12; GPR54; HOT7T175; KISS-1R; KISS1 receptor; G protein-coupled receptor 54; G-protein coupled receptor OT7T175; hypogonadotropin-1; kiSS-1 receptor; kisspeptins receptor; metastin receptor |
| GeneID | 84634 |
| Uni ProtID | Q969F8 |
| Chromosome Location | 19p13.3 |
| Function | neuropeptide binding; neuropeptide receptor activity; protein binding |
| Pathway | Class A/1 (Rhodopsin-like receptors); G alpha (q) signalling events; GPCR downstream signaling; GPCR ligand binding; Gastrin-CREB signalling pathway via PKC and MAPK; Neuroactive ligand-receptor interaction; Peptide ligand-binding receptors; |
| MIM | 604161 |
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The KISS1R stable cells responded beautifully to kisspeptin stimulation in our downstream signaling assays. Maintenance is very straightforward, and they retain their phenotypic characteristics perfectly even after extensive passaging. Highly satisfied with this purchase.
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