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Human KISS1R Stable Cell Line-CHO-K1

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RG1681

Host Cell :   CHO-K1 Size :   >1x106 frozen cells/vial

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Cell Line Information

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Gene Information

Cat. No. CSC-RG1681
Background The protein encoded by this gene is a galanin-like G protein-coupled receptor that binds metastin, a peptide encoded by the metastasis suppressor gene KISS1. The tissue distribution of the expressed gene suggests that it is involved in the regulation of endocrine function, and this is supported by the finding that this gene appears to play a role in the onset of puberty. Mutations in this gene have been associated with hypogonadotropic hypogonadism and central precocious puberty.
Target Gene KISS1R
Gene Species Homo sapiens (Human)
Host Cell CHO-K1
Host Cell Species Cricetulus griseus (Chinese hamster)
Source CHO-K1
Applications

1. Gene expression studies

2. Signaling pathway research

3. Drug screening and toxicology

4. Research on the mechanisms of GPCR-related diseases

Size >1x106 frozen cells/vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry Ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name KISS1R KISS1 receptor [ Homo sapiens ]
Gene Symbol KISS1R
Synonyms KISS1R; AXOR12; GPR54; HOT7T175; KISS-1R; KISS1 receptor; G protein-coupled receptor 54; G-protein coupled receptor OT7T175; hypogonadotropin-1; kiSS-1 receptor; kisspeptins receptor; metastin receptor
GeneID 84634
Uni ProtID Q969F8
Chromosome Location 19p13.3
Function neuropeptide binding; neuropeptide receptor activity; protein binding
Pathway Class A/1 (Rhodopsin-like receptors); G alpha (q) signalling events; GPCR downstream signaling; GPCR ligand binding; Gastrin-CREB signalling pathway via PKC and MAPK; Neuroactive ligand-receptor interaction; Peptide ligand-binding receptors;
MIM 604161
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Highly Responsive and Easy to Culture

The KISS1R stable cells responded beautifully to kisspeptin stimulation in our downstream signaling assays. Maintenance is very straightforward, and they retain their phenotypic characteristics perfectly even after extensive passaging. Highly satisfied with this purchase.

Japan

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