Human KCNQ3/KCNQ5 Stable Cell Line-HEK293

Name: Human KCNQ3/KCNQ5 Stable Cell Line-HEK293
SKU: CSC-RI0147
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0147

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0147
Description	This cell line is engineered to overexpress human KCNQ5.
Background	The human KCNQ3 and KCNQ5 genes encode the pore-forming subunits of Kv7.3 and Kv7.5, respectively. These subunits form heteromeric voltage-gated potassium channels that underlie M-current that regulates repetitive activity in neurons. Mutations in KCNQ3 that reduce M-current are responsible for some forms of benign familial neonatal seizure. Kv7.3/Kv7.5 channels expressed in neurons are therapeutic targets in seizure and cognitive impairment.
Target Gene	KCNQ5
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuKCNQ3/KCNQ5
Alias	KCNQ5, Kv7.5
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNQ5 potassium voltage-gated channel, KQT-like subfamily, member 5 [ Homo sapiens ]
Gene Symbol	KCNQ5
Synonyms	KCNQ5; potassium voltage-gated channel, KQT-like subfamily, member 5; potassium voltage-gated channel subfamily KQT member 5; Kv7.5; KQT-like 5; potassium channel protein; potassium channel subunit alpha KvLQT5; voltage-gated potassium channel subunit Kv7.5;
GeneID	56479
Uni ProtID	Q9NR82
mRNA Refseq	BC117359
Chromosome Location	6q14
Function	inward rectifier potassium channel activity; voltage-gated ion channel activity;
Pathway	Cholinergic synapse, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem; Voltage gated Potassium channels, organism-specific biosystem;
MIM	607357

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