Human KCND2/KCNIP2 Stable Cell Line-HEK293

Name: Human KCND2/KCNIP2 Stable Cell Line-HEK293
SKU: CSC-RI0139
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0139

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0139
Description	This cell line is engineered to overexpress human KCNIP2.
Background	The human KCND2 gene encodes the pore-forming subunit of Kv4.2, a voltage-gated potassium channel. The KCNIP2 gene encodes an auxiliary subunit of the channel. Kv4.2/KChIP2.2 channels expressed in neurons are therapeutic targets in nociceptive and inflammatory pain.
Target Gene	KCNIP2
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuKCND2/KCNIP2
Alias	KCNIP2, KCHIP2, MGC17241, DKFZp566L1246
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNIP2 Kv channel interacting protein 2 [ Homo sapiens ]
Gene Symbol	KCNIP2
Synonyms	KCHIP2
Gene Description	Kv channel interacting protein 2
Gene ID	30819
Uni Prot ID	B3KSZ5
m RNA Refseq	NM_173191.2
Protein Refseq	NP_775283.1
Chromosome Location	10q24
Function	A-type (transient outward) potassium channel activity; ER retention sequence binding; calcium ion binding; identical protein binding; protein N-terminus binding; protein binding;
MIM	604661

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