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Cat.No. : CSC-RI0035 Host Cell: CHO-K1
| Cat. No. | CSC-RI0035 |
| Description | This cell line is engineered to overexpress human KCNB1. |
| Background | Kv2.1 is expressed in the brain (cell bodies and dendrites of neurons but not axons, as well as Schwann cells), atria, ventricle, skeletal muscle, cochlea, retina, lung and pulmonary arteries. It is involved in maintaining membrane potential and modulating electrical excitability of both neurons and muscle cells. It is thought that Kv2.1 has a role in the muscle tone of the pulmonary system, as the expression Kv2.1 in pulmonary smooth muscle has been found to be reduced in chronic hypoxic pulmonary hypertension. |
| Gene | KCNB1 |
| Gene Species | Homo sapiens (Human) |
| Alias | KCNB1, DRK1, KV2.1, h-DRK1 |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: By optimizing transfection conditions and selecting appropriate promoters to ensure that the expression level of the KCNB1 gene in CHO cells is close to its physiological expression level in humans.
A: This cell line can be used for screening and evaluating drugs targeting the KCNB1 channel, especially those potentially affecting cardiac and nervous system functions.
A: It can be used to study the electrophysiological properties of the KCNB1 channel, including the dynamics of channel opening and closing, and its response to voltage changes.
A: Consideration should be given to transcriptional regulation, mRNA stability, and post-translational modifications of the protein, all of which can affect the expression of the KCNB1 gene.
A: It can be used to establish cellular models of cardiac diseases and certain neurodegenerative diseases, especially those related to dysfunctions of the KCNB1 channel.
A: Regularly change the culture medium, control cell density, and avoid over-passaging to maintain cell vitality and characteristics.
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