Human KCNB1 Stable Cell Line-CHO-K1

Name: Human KCNB1 Stable Cell Line-CHO-K1
SKU: CSC-RI0035
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0035

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0035
Description	This cell line is engineered to overexpress human KCNB1.
Background	Kv2.1 is expressed in the brain (cell bodies and dendrites of neurons but not axons, as well as Schwann cells), atria, ventricle, skeletal muscle, cochlea, retina, lung and pulmonary arteries. It is involved in maintaining membrane potential and modulating electrical excitability of both neurons and muscle cells. It is thought that Kv2.1 has a role in the muscle tone of the pulmonary system, as the expression Kv2.1 in pulmonary smooth muscle has been found to be reduced in chronic hypoxic pulmonary hypertension.
Target Gene	KCNB1
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuKCNB1
Alias	KCNB1, DRK1, KV2.1, h-DRK1
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNB1 potassium voltage-gated channel, Shab-related subfamily, member 1 [ Homo sapiens ]
Gene Symbol	KCNB1
Synonyms	DRK1; KV2.1; h-DRK1
Gene Description	potassium voltage-gated channel, Shab-related subfamily, member 1
GeneID	3745
Uni ProtID	Q14721
mRNA Refseq	NM_004975.2
Protein Refseq	NP_004966.1
Chromosome Location	20q13.2
Function	protein binding; voltage-gated potassium channel activity;
Pathway	Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Integration of energy metabolism, organism-specific biosystem; Metabolism, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem; Regulation of Insulin Secretion, organism-specific biosystem; Regulation of Insulin Secretion by Glucagon-like Peptide-1, organism-specific biosystem;
MIM	600397

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Q & A

Customer Reviews

Customer Q&As

How is the physiological relevance of KCNB1 gene expression ensured in the Human KCNB1 Stable Cell Line-CHO?

A: By optimizing transfection conditions and selecting appropriate promoters to ensure that the expression level of the KCNB1 gene in CHO cells is close to its physiological expression level in humans.

What are the advantages of using the Human KCNB1 Stable Cell Line-CHO in pharmacological research?

A: This cell line can be used for screening and evaluating drugs targeting the KCNB1 channel, especially those potentially affecting cardiac and nervous system functions.

What are the applications of the Human KCNB1 Stable Cell Line-CHO in electrophysiological experiments?

A: It can be used to study the electrophysiological properties of the KCNB1 channel, including the dynamics of channel opening and closing, and its response to voltage changes.

What factors should be considered when studying gene expression using the Human KCNB1 Stable Cell Line-CHO?

A: Consideration should be given to transcriptional regulation, mRNA stability, and post-translational modifications of the protein, all of which can affect the expression of the KCNB1 gene.

What are the applications of the Human KCNB1 Stable Cell Line-CHO in simulating disease models?

A: It can be used to establish cellular models of cardiac diseases and certain neurodegenerative diseases, especially those related to dysfunctions of the KCNB1 channel.

How to avoid overgrowth or senescence of cells when using the Human KCNB1 Stable Cell Line-CHO?

A: Regularly change the culture medium, control cell density, and avoid over-passaging to maintain cell vitality and characteristics.

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