Human KCNA7 Stable Cell Line-HEK293

Name: Human KCNA7 Stable Cell Line-HEK293
SKU: CSC-RI0134
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0134

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0134
Description	This cell line is engineered to overexpress human KCNA7.
Background	Kv1.7 is a voltage-gated, K+-selective channel expressed in the placenta, amnion, skeletal muscle, and pulmonary artery.
Target Gene	KCNA7
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuKCNA7
Alias	KCNA7, HAK6, Kv1.7, KV1.7
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KCNA7 potassium voltage-gated channel, shaker-related subfamily, member 7 [ Homo sapiens ]
Gene Symbol	KCNA7
Synonyms	HAK6; KV1.7
Gene Description	potassium voltage-gated channel, shaker-related subfamily, member 7
GeneID	3743
Uni ProtID	Q96RP8
mRNA Refseq	NM_031886.2
Protein Refseq	NP_114092.2
Chromosome Location	19q13.3
Function	voltage-gated potassium channel activity;
Pathway	Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem; Voltage gated Potassium channels, organism-specific biosystem;
MIM	176268

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