Human HCN1 Stable Cell Line-HEK293

Name: Human HCN1 Stable Cell Line-HEK293
SKU: CSC-RI0011
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0011

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0011
Description	This cell line is engineered to overexpress human HCN1.
Background	HCN channels contribute to rhythmic spontaneous electrical activity in the heart and CNS. Interference with HCN1 activity can lead to various forms of arrhythmias. The spontaneous neuronal activity of HCN channels have also been implicated in the development and maintenance of a wide range of pain states and an inherited loss of HCN1 channel expression is involved in generalized idiopathic epileptic seizures.
Target Gene	HCN1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuHCN1
Alias	HCN1, BCNG1, BCNG 1, HAC 2, HAC-2, BCNG-1
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	HCN1 hyperpolarization activated cyclic nucleotide-gated potassium channel 1 [ Homo sapiens ]
Gene Symbol	HCN1
Synonyms	BCNG1; HAC-2; BCNG-1
GeneID	15165
Uni ProtID	O60741
mRNA Refseq	NM_021072.3
Protein Refseq	NP_066550.2
Chromosome Location	5p12
Function	cAMP binding; potassium channel activity; sodium channel activity; voltage-gated potassium channel activity;
Pathway	HCN channels, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem;
MIM	602780

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Q & A

Customer Reviews

Customer Q&As

How was the Human HCN1 Stable Cell Line-HEK293 established?

A: The cell line was established by stably transfecting the human HCN1 (hyperpolarization-activated cyclic nucleotide-gated channel 1, found in heart and neural tissues) gene into HEK293 cells.

What is the application of the Human HCN1 Stable Cell Line-HEK293 in cardiology research?

A: This cell line can be used to study the role of HCN1 in cardiac rhythm regulation and heart diseases.

What is the recommended usage amount for the Human HCN1 Stable Cell Line-HEK293?

A: The recommended usage amount depends on the type of experiment, generally involving seeding an appropriate number of cells in a culture plate to achieve the desired cell density.

What precautions should be taken when using the Human HCN1 Stable Cell Line-HEK293?

A: Maintain a sterile operating environment and regularly check the growth of the cells and the expression of the HCN1 gene.

What are the advantages of using the Human HCN1 Stable Cell Line-HEK293 for neuroscience research?

A: It can be used to study the role of HCN1 in neural signaling and neurological diseases, such as epilepsy.

What is the application of this cell line in drug screening and evaluation?

A: It can be used for screening drugs that may affect HCN1 channel activity, evaluating their potential effects on treating cardiac and neural disorders.

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