Human HCN1 Stable Cell Line-HEK293, tetracycline-inducible

Name: Human HCN1 Stable Cell Line-HEK293, tetracycline-inducible
SKU: CSC-RI0099
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0099

Host Cell : HEK293, tetracycline-inducible Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0099
Description	This cell line is engineered to overexpress human HCN1.
Background	Human HCN1 hyperpolarization-gated ion channel-expressing, stable replicating cell line.
Target Gene	HCN1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuHCN1, tetracycline-inducible
Alias	HCN1, BCNG1, BCNG 1, HAC 2, HAC-2, BCNG-1
Host Cell	HEK293, tetracycline-inducible
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	HCN1 hyperpolarization activated cyclic nucleotide-gated potassium channel 1 [ Homo sapiens ]
Gene Symbol	HCN1
Synonyms	BCNG1; HAC-2; BCNG-1
GeneID	15165
Uni ProtID	O60741
mRNA Refseq	NM_021072.3
Protein Refseq	NP_066550.2
Chromosome Location	5p12
Function	cAMP binding; potassium channel activity; sodium channel activity; voltage-gated potassium channel activity;
Pathway	HCN channels, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem;
MIM	602780

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