Human HCN2 Stable Cell Line-HEK293

Name: Human HCN2 Stable Cell Line-HEK293
SKU: CSC-RI0012
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0012

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0012
Description	This cell line is engineered to overexpress human HCN2.
Background	Hyperpolarization-activated, cyclic nucleotide-modulated (HCN) channels contribute to rhythmic spontaneous electrical activity in the heart and CNS. Interference with HCN2 activity can lead to various forms of cardiac arrhythmias, due to dysfunction of the sinus node. The predominant role of HCN2 in these cells may be the stabilization of a normal diastolic membrane potential.
Target Gene	HCN2
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuHCN2
Alias	HCN2, BCNG2, HAC 1, HAC-1, BCNG-2
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	HCN2 hyperpolarization activated cyclic nucleotide-gated potassium channel 2 [ Homo sapiens ]
Gene Symbol	HCN2
Synonyms	BCNG2; HAC-1; BCNG-2
Gene Description	hyperpolarization activated cyclic nucleotide-gated potassium channel 2
GeneID	15166
Uni ProtID	Q9UL51
mRNA Refseq	NM_001194.3
Protein Refseq	NP_001185.3
Chromosome Location	19p13.3
Function	cAMP binding; cation channel activity; protein complex scaffold; sodium channel activity; voltage-gated potassium channel activity;
Pathway	HCN channels, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem;
MIM	602781

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