Human HCN3 Stable Cell Line-HEK293

Name: Human HCN3 Stable Cell Line-HEK293
SKU: CSC-RI0013
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0013

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0013
Description	This cell line is engineered to overexpress human HCN3.
Target Gene	HCN3
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuHCN3
Alias	HCN3, KIAA1535, MGC131493
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	HCN3 hyperpolarization activated cyclic nucleotide-gated potassium channel 3 [ Homo sapiens ]
Gene Symbol	HCN3
Synonyms	HCN3; hyperpolarization activated cyclic nucleotide-gated potassium channel 3; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3; KIAA1535; MGC131493;
GeneID	57657
Uni ProtID	Q9P1Z3
mRNA Refseq	BC028024
Chromosome Location	1q21.2
Function	cAMP binding; nucleotide binding; sodium channel activity; voltage-gated ion channel activity; voltage-gated potassium channel activity;
Pathway	HCN channels, organism-specific biosystem; Neuronal System, organism-specific biosystem; Potassium Channels, organism-specific biosystem;
MIM	609973

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Background

Q & A

Customer Reviews

Four different hyperpolarization-activated, cyclic nucleotide-modulated (HCN) channel isoforms have been cloned (HCN1-4). They can be distinguished by differing sensitivity to modulators such as cAMP, different V1/2 values of activation and different activation kinetics. Even though they are structurally similar to Kv channels they are activated by strong hyperpolarizing voltages rather than depolarizing voltages. As such the channels are active at or around the resting potential and contribute to pacemaker activity both in the heart and CNS. There is considerable overlap in the expression of these isoforms in different tissues and it is highly likely that they can form heteromultimers. Altered HCN channel expression has been implicated in increased neuronal hyperexcitability following seizures and may represent a novel drug target for the treatment of seizure-evoked epilepsy. HCN channels also appear to have a role in some models of neuropathic pain where the spontaneous firing of damaged nerves and therefore the sensation of pain can be alleviated by the selective HCN blocker ZD7288.

Customer Q&As

How was the Human HCN3 Stable Cell Line-HEK293 established?

A: The cell line was established by stably transfecting the human HCN3 (hyperpolarization-activated cyclic nucleotide-gated channel 3) gene into HEK293 cells.

What is the application of the Human HCN3 Stable Cell Line-HEK293 in cardiac physiology research?

A: This cell line can be used to study the role of HCN3 in cardiac rhythm formation and regulation.

What are the main uses of the Human HCN3 Stable Cell Line-HEK293 in neuroscience research?

A: It can be used to study the role of HCN3 in neuronal excitability and signal transduction.

What experimental conditions should be noted when using the Human HCN3 Stable Cell Line-HEK293?

A: The cells should be cultured at 37°C in a 5% CO2 environment, maintaining appropriate cell density and nutritional conditions.

What are the advantages of using the Human HCN3 Stable Cell Line-HEK293 for drug screening?

A: It can be used for high-throughput screening of drugs affecting HCN3 channel activity, aiding in the discovery of new treatments for cardiac and neurological diseases.

How can genetic stability be ensured while maintaining the Human HCN3 Stable Cell Line-HEK293?

A: Regular phenotypic and genotypic testing should be conducted to ensure stable expression of the HCN3 gene and consistency in cell characteristics.

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