Human HCN3 Stable Cell Line-HEK293

Name: Human HCN3 Stable Cell Line-HEK293
SKU: CSC-RI0013

Cat.No. : CSC-RI0013 Host Cell: HEK293

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RI0013
Description	This cell line is engineered to overexpress human HCN3.
Gene	HCN3
Gene Species	Homo sapiens (Human)
Alias	HCN3, KIAA1535, MGC131493
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Morphology	Epithelial
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

Publications

Q & A

Customer Reviews

Four different hyperpolarization-activated, cyclic nucleotide-modulated (HCN) channel isoforms have been cloned (HCN1-4). They can be distinguished by differing sensitivity to modulators such as cAMP, different V1/2 values of activation and different activation kinetics. Even though they are structurally similar to Kv channels they are activated by strong hyperpolarizing voltages rather than depolarizing voltages. As such the channels are active at or around the resting potential and contribute to pacemaker activity both in the heart and CNS. There is considerable overlap in the expression of these isoforms in different tissues and it is highly likely that they can form heteromultimers. Altered HCN channel expression has been implicated in increased neuronal hyperexcitability following seizures and may represent a novel drug target for the treatment of seizure-evoked epilepsy. HCN channels also appear to have a role in some models of neuropathic pain where the spontaneous firing of damaged nerves and therefore the sensation of pain can be alleviated by the selective HCN blocker ZD7288.

Customer Q&As

How was the Human HCN3 Stable Cell Line-HEK293 established?

A: The cell line was established by stably transfecting the human HCN3 (hyperpolarization-activated cyclic nucleotide-gated channel 3) gene into HEK293 cells.

What is the application of the Human HCN3 Stable Cell Line-HEK293 in cardiac physiology research?

A: This cell line can be used to study the role of HCN3 in cardiac rhythm formation and regulation.

What are the main uses of the Human HCN3 Stable Cell Line-HEK293 in neuroscience research?

A: It can be used to study the role of HCN3 in neuronal excitability and signal transduction.

What experimental conditions should be noted when using the Human HCN3 Stable Cell Line-HEK293?

A: The cells should be cultured at 37°C in a 5% CO2 environment, maintaining appropriate cell density and nutritional conditions.

What are the advantages of using the Human HCN3 Stable Cell Line-HEK293 for drug screening?

A: It can be used for high-throughput screening of drugs affecting HCN3 channel activity, aiding in the discovery of new treatments for cardiac and neurological diseases.

How can genetic stability be ensured while maintaining the Human HCN3 Stable Cell Line-HEK293?

A: Regular phenotypic and genotypic testing should be conducted to ensure stable expression of the HCN3 gene and consistency in cell characteristics.

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