Human GRIN1/GRIN2B Stable Cell Line-HEK293

Name: Human GRIN1/GRIN2B Stable Cell Line-HEK293
SKU: CSC-RI0175
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0175

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0175
Description	This cell line is engineered to overexpress human GRIN2B.
Background	The human GRIN1/GRIN2B genes encode the cationic-selective, n-methyl-d-aspartic acid receptor-channel expressed in the CNS. NMDA receptors are therapeutic targets in treatment of neurological disorders including seizure, pain, and Alzheimer"s disease.
Target Gene	GRIN2B
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuGRIN1/GRIN2B
Alias	GRIN2B, NMDAR2B, GluN2B, NR3, MRD6, NR2B, hNR3, MGC142178, MGC142180
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GRIN2B glutamate receptor, ionotropic, N-methyl D-aspartate 2B [ Homo sapiens ]
Gene Symbol	GRIN2B
Synonyms	MRD6; NR2B; hNR3; GluN2B; NMDAR2B
Gene Description	glutamate receptor, ionotropic, N-methyl D-aspartate 2B
GeneID	2904
Uni ProtID	Q13224
mRNA Refseq	NM_000834.3
Protein Refseq	NP_000825.2
Chromosome Location	12p12
Function	D2 dopamine receptor binding; N-methyl-D-aspartate selective glutamate receptor activity; beta-catenin binding; calcium channel activity; cell adhesion molecule binding; drug binding; extracellular-glutamate-gated ion channel activity; glycine binding; ionotropic glutamate receptor binding; neurotransmitter binding; protein binding; protein heterodimerization activity; zinc ion binding;
Pathway	Activation of NMDA receptor upon glutamate binding and postsynaptic events, organism-specific biosystem; Alcoholism, organism-specific biosystem; Alcoholism, conserved biosystem; Alzheimers disease, organism-specific biosystem; Alzheimers disease, conserved biosystem; Alzheimers Disease, organism-specific biosystem; Amphetamine addiction, organism-specific biosystem;
MIM	138252

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