Human GHSR-SNAP Stable Cell Line-HEK293

Name: Human GHSR-SNAP Stable Cell Line-HEK293
SKU: CSC-RG0049

Cat.No. : CSC-RG0049 Host Cell: HEK293

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RG0049
Gene	GHSR
Gene Species	Homo sapiens (Human)
Alias	GHSR, GHRP, GHS-R
Host Cell	HEK293
Species	Homo sapiens (Human)
Morphology	Epithelial
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

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Customer Reviews

This gene encodes a member of the G-protein coupled receptor family. The encoded protein may play a role in energy homeostasis and regulation of body weight. Two identified transcript variants are expressed in several tissues and are evolutionary conserved in fish and swine. One transcript, 1a, excises an intron and encodes the functional protein; this protein is the receptor for the Ghrelin ligand and defines a neuroendocrine pathway for growth hormone release. The second transcript (1b) retains the intron and does not function as a receptor for Ghrelin; however, it may function to attenuate activity of isoform 1a. Mutations in this gene are associated with autosomal idiopathic short stature.

Customer Q&As

What is the primary function of the Human GHSR-SNAP Stable Cell Line-HEK293 in research settings?

A: The Human GHSR-SNAP Stable Cell Line-HEK293 is primarily used for studying the signaling pathways and functions of the growth hormone secretagogue receptor (GHSR). This cell line is engineered to express the GHSR, which allows researchers to investigate how ligands bind to the receptor and trigger downstream signaling events, such as the release of growth hormone.

How does the SNAP tag in the Human GHSR-SNAP Stable Cell Line-HEK293 facilitate protein-protein interactions studies?

A: The SNAP tag is a small protein tag that enables the specific labeling of proteins of interest with biotin or other chemical probes. In the context of the Human GHSR-SNAP Stable Cell Line-HEK293, this tag allows researchers to selectively label the GHSR protein, which can then be used to study its interactions with other proteins in the cell, providing insights into the receptor's role in cellular processes.

What are the advantages of using the Human GHSR-SNAP Stable Cell Line-HEK293 for high-throughput screening?

A: The Human GHSR-SNAP Stable Cell Line-HEK293 offers several advantages for high-throughput screening (HTS). It provides a consistent and reproducible system for testing the effects of various compounds on GHSR activity. The SNAP tag also simplifies the detection process, as it can be used with a variety of detection methods, making the screening process more efficient and versatile.

How does the Human GHSR-SNAP Stable Cell Line-HEK293 contribute to the understanding of growth hormone regulation?

A: This cell line contributes to the understanding of growth hormone regulation by providing a platform to study the mechanisms of GHSR activation and deactivation. Researchers can use this cell line to investigate how different ligands affect receptor function and how this, in turn, influences the release of growth hormone, which is crucial for growth and metabolism.

How does the Human GHSR-SNAP Stable Cell Line-HEK293 aid in the development of new therapeutics targeting the GHSR?

A: The Human GHSR-SNAP Stable Cell Line-HEK293 serves as a model system for testing the efficacy and safety of potential drugs that target the GHSR. By screening compounds in this cell line, researchers can identify potential leads that modulate GHSR activity, which could then be further developed into therapeutics for conditions related to growth hormone dysregulation.

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