Cat.No. : CSC-RG0015 Host Cell: U2OS
| Cat. No. | CSC-RG0015 |
| Background | Muscarinic acetylcholine receptors are G protein-coupled receptors. M1, M3, M5 receptors couple to G proteins of the Gq/11 family, which activate phospholipase C. M2 and M4 receptors couple to Gi/o-type G proteins that inhibit adenylyl cyclase activity. Muscarinic receptors control many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor have not been fully explored; however, stimulation of this receptor is known to effectively decrease cAMP levels and downregulate the activity of protein kinase A (PKA). |
| Gene | CHRM5 |
| Gene Species | Homo sapiens (Human) |
| Alias | CHRM5, HM5 |
| Host Cell | U2OS |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CHRM5 Stable Cell Line-U2OS, established by stably expressing the human muscarinic acetylcholine receptor 5 (CHRM5) in U2OS cells, plays a crucial role in studying the function of the cholinergic system in cognitive functions and neurodegenerative diseases. Scientists can simulate and investigate the activity changes of CHRM5 receptors and how these changes affect neural signal transmission and cellular functions.
A: By conducting drug screening and testing on the Human CHRM5 Stable Cell Line-U2OS, researchers can evaluate the selectivity of potential drugs for the CHRM5 receptor. This is significant for developing drugs targeting the CHRM5 receptor, such as those for treating Alzheimer's disease. This cell line helps scientists understand how drugs interact with the CHRM5 receptor and assess their potential therapeutic effects and side effects.
A: The Human CHRM5 Stable Cell Line-U2OS can be used to study the role of CHRM5 receptors in neurodegenerative diseases, such as Alzheimer's disease. This cell line provides a platform for investigating the expression and functional changes of CHRM5 receptors in these diseases, as well as potential therapeutic interventions.
A: The Human CHRM5 Stable Cell Line-U2OS is crucial for understanding the role of CHRM5 receptors in cognitive functions. This cell line helps scientists investigate how CHRM5 receptors influence learning and memory processes, which is significant for developing drugs to improve cognitive function.
A: The Human CHRM5 Stable Cell Line-U2OS can be used to study the role of CHRM5 receptors in neural signal transmission, including their roles at the presynaptic and postsynaptic sites. This cell line helps scientists uncover the specific mechanisms of action of CHRM5 receptors in neural signal transmission, which is crucial for understanding the complex functions of the nervous system.
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