Human CHRM5 Stable Cell Line-U2OS

Name: Human CHRM5 Stable Cell Line-U2OS
SKU: CSC-RG0015
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0015

Host Cell : U2OS Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0015
Background	Muscarinic acetylcholine receptors are G protein-coupled receptors. M1, M3, M5 receptors couple to G proteins of the Gq/11 family, which activate phospholipase C. M2 and M4 receptors couple to Gi/o-type G proteins that inhibit adenylyl cyclase activity. Muscarinic receptors control many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor have not been fully explored; however, stimulation of this receptor is known to effectively decrease cAMP levels and downregulate the activity of protein kinase A (PKA).
Target Gene	CHRM5
Gene Species	Homo sapiens (Human)
Abbr	U2OS-HuCHRM5
Alias	CHRM5, HM5
Host Cell	U2OS
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CHRM5 cholinergic receptor, muscarinic 5 [ Homo sapiens ]
Gene Symbol	CHRM5
Synonyms	CHRM5; cholinergic receptor, muscarinic 5; muscarinic acetylcholine receptor M5; acetylcholine receptor; muscarinic 5; acetylcholine receptor, muscarinic 5; HM5; MGC41838;
GeneID	1133
Uni ProtID	P08912
mRNA Refseq	BC041805
Chromosome Location	15q26
Function	G-protein coupled acetylcholine receptor activity; G-protein coupled receptor activity; phosphatidylinositol phospholipase C activity; receptor activity; signal transducer activity;
Pathway	Amine ligand-binding receptors, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Cholinergic synapse, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem;
MIM	118496

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Customer Q&As

What is the role of the Human CHRM5 Stable Cell Line-U2OS in studying the function of the cholinergic system?

A: The Human CHRM5 Stable Cell Line-U2OS, established by stably expressing the human muscarinic acetylcholine receptor 5 (CHRM5) in U2OS cells, plays a crucial role in studying the function of the cholinergic system in cognitive functions and neurodegenerative diseases. Scientists can simulate and investigate the activity changes of CHRM5 receptors and how these changes affect neural signal transmission and cellular functions.

How is the Human CHRM5 Stable Cell Line-U2OS used in drug development to assess the selectivity of drugs for the CHRM5 receptor?

A: By conducting drug screening and testing on the Human CHRM5 Stable Cell Line-U2OS, researchers can evaluate the selectivity of potential drugs for the CHRM5 receptor. This is significant for developing drugs targeting the CHRM5 receptor, such as those for treating Alzheimer's disease. This cell line helps scientists understand how drugs interact with the CHRM5 receptor and assess their potential therapeutic effects and side effects.

How is the Human CHRM5 Stable Cell Line-U2OS applied in studying the role of CHRM5 receptors in neurodegenerative diseases?

A: The Human CHRM5 Stable Cell Line-U2OS can be used to study the role of CHRM5 receptors in neurodegenerative diseases, such as Alzheimer's disease. This cell line provides a platform for investigating the expression and functional changes of CHRM5 receptors in these diseases, as well as potential therapeutic interventions.

How is the Human CHRM5 Stable Cell Line-U2OS applied in studying the role of CHRM5 receptors in cognitive function?

A: The Human CHRM5 Stable Cell Line-U2OS is crucial for understanding the role of CHRM5 receptors in cognitive functions. This cell line helps scientists investigate how CHRM5 receptors influence learning and memory processes, which is significant for developing drugs to improve cognitive function.

How is the Human CHRM5 Stable Cell Line-U2OS applied in studying the role of CHRM5 receptors in neural signal transmission?

A: The Human CHRM5 Stable Cell Line-U2OS can be used to study the role of CHRM5 receptors in neural signal transmission, including their roles at the presynaptic and postsynaptic sites. This cell line helps scientists uncover the specific mechanisms of action of CHRM5 receptors in neural signal transmission, which is crucial for understanding the complex functions of the nervous system.

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