Cat.No. : CSC-RG0060 Host Cell: CHO-K1
| Cat. No. | CSC-RG0060 |
| Background | The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. |
| Gene | CHRM3 |
| Gene Species | Homo sapiens (Human) |
| Alias | CHRM3, HM3, EGBRS |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CHRM3 Stable Cell Line-CHO (Chinese Hamster Ovary) is engineered to stably express the human muscarinic acetylcholine receptor subtype 3 (CHRM3). This cell line is ideal for studying receptor function due to its high receptor expression levels, which allow for sensitive detection of ligand binding and signaling events. Additionally, CHO cells are known for their low endogenous receptor background, reducing interference from native receptors.
A: This cell line provides a reliable and reproducible model for high-throughput screening (HTS) of potential drug candidates. By measuring the effects of compounds on CHRM3 receptor activity, researchers can identify lead compounds with potential therapeutic value. The cell line's stability and reproducibility ensure consistent results across different experiments, which is crucial for drug discovery and development.
A: This cell line enables researchers to investigate the pharmacological properties of CHRM3, such as its agonist and antagonist binding affinities, as well as its signaling pathways. The controlled expression of CHRM3 allows for the study of receptor activation and desensitization, which are critical aspects of receptor pharmacology.
A: Unlike primary cells, which can have complex and variable receptor expression profiles, the Human CHRM3 Stable Cell Line-CHO offers a uniform and controlled environment for receptor expression. This allows for more accurate and reproducible results, which is particularly important for drug screening and mechanistic studies.
A: The advantages include high receptor expression, which enhances assay sensitivity, and the ability to perform functional assays in a cell line that is easy to culture and manipulate. This cell line also allows for the study of receptor function in the absence of other confounding factors present in primary cells or tissues.
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