Human CHRM3 Stable Cell Line-CHO-K1

Name: Human CHRM3 Stable Cell Line-CHO-K1
SKU: CSC-RG0060
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0060

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

Cat. No.	CSC-RG0060
Background	The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue.
Target Gene	CHRM3
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuCHRM3
Alias	CHRM3, HM3, EGBRS
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent
Morphology	Epithelial-like

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CHRM3 cholinergic receptor, muscarinic 3 [ Homo sapiens ]
Gene Symbol	CHRM3
Synonyms	HM3; EGBRS
Gene Description	cholinergic receptor, muscarinic 3
GeneID	1131
Uni ProtID	P20309
mRNA Refseq	NM_000740.2
Protein Refseq	NP_000731.1
Chromosome Location	1q43
Function	G-protein coupled acetylcholine receptor activity; acetylcholine binding; drug binding; phosphatidylinositol phospholipase C activity; receptor activity;
Pathway	Amine ligand-binding receptors, organism-specific biosystem; Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Cholinergic synapse, organism-specific biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem;
MIM	118494

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What are the key characteristics of the Human CHRM3 Stable Cell Line-CHO that make it suitable for studying muscarinic receptor function?

A: The Human CHRM3 Stable Cell Line-CHO (Chinese Hamster Ovary) is engineered to stably express the human muscarinic acetylcholine receptor subtype 3 (CHRM3). This cell line is ideal for studying receptor function due to its high receptor expression levels, which allow for sensitive detection of ligand binding and signaling events. Additionally, CHO cells are known for their low endogenous receptor background, reducing interference from native receptors.

How does the Human CHRM3 Stable Cell Line-CHO contribute to the development of drugs targeting the muscarinic system?

A: This cell line provides a reliable and reproducible model for high-throughput screening (HTS) of potential drug candidates. By measuring the effects of compounds on CHRM3 receptor activity, researchers can identify lead compounds with potential therapeutic value. The cell line's stability and reproducibility ensure consistent results across different experiments, which is crucial for drug discovery and development.

How does the Human CHRM3 Stable Cell Line-CHO facilitate the study of receptor pharmacology?

A: This cell line enables researchers to investigate the pharmacological properties of CHRM3, such as its agonist and antagonist binding affinities, as well as its signaling pathways. The controlled expression of CHRM3 allows for the study of receptor activation and desensitization, which are critical aspects of receptor pharmacology.

How does the Human CHRM3 Stable Cell Line-CHO differ from primary cell models in terms of receptor expression?

A: Unlike primary cells, which can have complex and variable receptor expression profiles, the Human CHRM3 Stable Cell Line-CHO offers a uniform and controlled environment for receptor expression. This allows for more accurate and reproducible results, which is particularly important for drug screening and mechanistic studies.

What are the advantages of using the Human CHRM3 Stable Cell Line-CHO for functional assays?

A: The advantages include high receptor expression, which enhances assay sensitivity, and the ability to perform functional assays in a cell line that is easy to culture and manipulate. This cell line also allows for the study of receptor function in the absence of other confounding factors present in primary cells or tissues.

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