Human CD80 Stable Cell Line - GS-C1

Name: Human CD80 Stable Cell Line - GS-C1
SKU: CSC-RO0232
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0232

Host Cell : GS-C1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0232
Description	This cell line is engineered to stably overexpress the human CD80 in GS-C1 cells.
Introduction	GS-C1/CD80 cell line stably expresses human CD80 molecule. The expression of CD80 in this cell line has been validated through flow cytometry. The functional validation of this cell line has been measured by cell-based anti-CTLA4 activity assay.
Target Gene	CD80
Gene Species	Homo sapiens (Human)
Host Cell	GS-C1
Host Cell Species	Homo sapiens (Human)
Source	GS-C1
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CD80 CD80 molecule [ Homo sapiens ]
Gene Symbol	CD80
Synonyms	B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Gene Description	CD80 molecule
Gene ID	941
Uni Prot ID	A0N0P2
m RNA Refseq	NM_005191.3
Protein Refseq	NP_005182.1
Chromosome Location	3q13.3-q21
Function	coreceptor activity; protein binding;
Pathway	Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem;
MIM	112203

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Q & A

Customer Reviews

Customer Q&As

How does the Human CD80 Stable Cell Line - GS-C1 contribute to the understanding of T cell activation and co-stimulation?

A: The Human CD80 Stable Cell Line - GS-C1 is instrumental in studying the role of CD80 (B7-1) in T cell activation and co-stimulation. CD80 is a critical co-stimulatory molecule that interacts with CD28 on T cells, enhancing their activation. This cell line allows researchers to investigate the molecular mechanisms of T cell activation and the potential therapeutic targets for autoimmune diseases and cancer immunotherapy.

What are the unique features of the Human CD80 Stable Cell Line - GS-C1 that make it suitable for immunological research?

A: The unique features of the Human CD80 Stable Cell Line - GS-C1 include its stable expression of CD80, which enables researchers to perform a wide range of immunological studies, such as investigating the interaction between CD80 and its receptors, its role in antigen presentation, and its involvement in regulatory T cell function.

How can the Human CD80 Stable Cell Line - GS-C1 be utilized in the development of novel immunotherapies?

A: The cell line can be used in high-throughput screening assays to identify compounds that modulate CD80 expression or function. This could lead to the discovery of new drugs that enhance or inhibit T cell responses, which are crucial for the development of immunotherapies for various diseases, including cancer and autoimmune disorders.

How does the Human CD80 Stable Cell Line - GS-C1 contribute to the understanding of CD80's role in immune tolerance and autoimmunity?

A: The cell line can be used to investigate how CD80 contributes to immune tolerance by studying its interactions with regulatory T cells and other immune cells. This can help elucidate the mechanisms behind autoimmune diseases where CD80-mediated co-stimulation may be dysregulated.

What are the ethical implications of using the Human CD80 Stable Cell Line - GS-C1 in research?

A: While the use of cell lines like GS-C1 does not raise the same ethical concerns as primary human tissue, researchers must still adhere to ethical guidelines for cell culture, including obtaining informed consent for the original cell source and ensuring the proper disposal of cell lines.

How can the Human CD80 Stable Cell Line - GS-C1 be used to study the impact of genetic variations in the CD80 gene on immune responses?

A: The cell line can serve as a platform for introducing specific genetic variations into the CD80 gene, allowing researchers to assess how these variations affect CD80 function and the resulting immune responses. This can provide insights into the genetic basis of individual differences in immune responses.

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