Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-2695 Host Cell : HEK293
| Cat. No. | CLOE-2695 |
| Introduction | This cell lysate overexpresses mouse Cd80 (Met1-Lys245) with a C-terminal polyhistidine tag. Purification of target protein from this cell lysate has been verified. |
| Target Gene | Cd80 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | Cd80 CD80 antigen [ Mus musculus ] |
| Gene Symbol | Cd80 |
| Synonyms | B71; Ly53; TSA1; Cd28l; Ly-53; MIC17 |
| Gene Description | CD80 antigen |
| Gene ID | 12519 |
| Uni Prot ID | Q00609 |
| m RNA Refseq | NM_009855.2 |
| Protein Refseq | NP_033985.3 |
| Chromosome Location | 16 B5; 16 28.0 cM |
| Function | protein binding; |
| Pathway | Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem; |
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