Human CD80(His/Fc) HEK293 Cell Lysate

Name: Human CD80(His/Fc) HEK293 Cell Lysate
SKU: CLOE-1258
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1258 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1258
Introduction	This cell lysate over-expresses the extracellular domain of human CD80 (NP_005182.1) (Met 1-Asn 242) fused with a polyhistidine tag and the Fc region of human IgG1 at the C-terminus. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	CD80
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	CD80 CD80 molecule [ Homo sapiens ]
Gene Symbol	CD80
Synonyms	B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Gene Description	CD80 molecule
Gene ID	941
Uni Prot ID	A0N0P2
m RNA Refseq	NM_005191.3
Protein Refseq	NP_005182.1
Chromosome Location	3q13.3-q21
Function	coreceptor activity; protein binding;
Pathway	Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem;
MIM	112203

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