Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CLOE-1249 Host Cell : HEK293
| Cat. No. | CLOE-1249 |
| Introduction | This cell lysate over-expresses the extracellular domain of the human CD80 (NP_005182.1) (Met 1-Asn 242) fused with five amini acids (DDDDK) at the C-terminus. Purification of target protein (s) from this cell lysate has been verified. |
| Target Gene | CD80 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications | Positive control for Western Blot (WB), antibody verification, protein purification |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Storage | Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided. |
| Shipping | Ice package |
| Gene Name | CD80 CD80 molecule [ Homo sapiens ] |
| Gene Symbol | CD80 |
| Synonyms | B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1 |
| Gene Description | CD80 molecule |
| Gene ID | 941 |
| Uni Prot ID | A0N0P2 |
| m RNA Refseq | NM_005191.3 |
| Protein Refseq | NP_005182.1 |
| Chromosome Location | 3q13.3-q21 |
| Function | coreceptor activity; protein binding; |
| Pathway | Adaptive Immune System, organism-specific biosystem; Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; CD28 co-stimulation, organism-specific biosystem; CD28 dependent PI3K/Akt signaling, organism-specific biosystem; |
| MIM | 112203 |
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