Human CCR2 Stable Cell Line-CHO-K1

Name: Human CCR2 Stable Cell Line-CHO-K1
SKU: CSC-RG0823

Cat.No. : CSC-RG0823 Host Cell: CHO-K1

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RG0823
Gene	CCR2
Gene Species	Homo sapiens (Human)
Alias	CCR2, CMKBR2, CC CKR 2, CD192, CKR2, FLJ78302, MCP 1 R, CCR2A, CCR2B, CKR2A, CKR2B, MCP-1-R, CC-CKR-2
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Morphology	Epithelial-like
Stability	Validated for at least 10 passages
Application	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Chemokines are a family of low molecular weight secreted proteins that act as leukocyte specifie chemoattractants. There are over 40 known chemokines which can be divided into four families: CXC, CC, C and CX3C. The CC-chemokines MCP-1,2,3,4 bind and activate CCR2 receptors. CCR2 receptors belong to the GPCR family and couple to the Gi signal pathway. CCR2 receptors are expressed on monocytes, macrophages, basophils, mast cells, T lymphocytes, NK cells and dendritic cells. There are two alternatively spliced forms of CCR2 receptors CCR2A and CCR2B, which differ only in their carboxyl-terminal tails. CCR2 receptors plays important roles in the recruitment of monocytes/macrophages, T cells and relates to many diseases such as inflammation, HIV, and pulmonary fibrosis.

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