Human CCKAR-SNAP Stable Cell Line-CHO

Name: Human CCKAR-SNAP Stable Cell Line-CHO
SKU: CSC-RG0045
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0045

Host Cell : CHO Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0045
Background	This gene encodes a G-protein coupled receptor that binds non-sulfated members of the cholecystokinin (CCK) family of peptide hormones. This receptor is a major physiologic mediator of pancreatic enzyme secretion and smooth muscle contraction of the gallbladder and stomach. In the central and peripheral nervous system this receptor regulates satiety and the release of beta-endorphin and dopamine.
Target Gene	CCKAR
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuCCKAR-SNAP
Alias	CCKAR, CCK-AR, CCK-A, CCKRA, CCK1-R
Host Cell	CHO
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CCKAR cholecystokinin A receptor [ Homo sapiens ]
Gene Symbol	CCKAR
Synonyms	CCK-A; CCKRA; CCK1-R
Gene Description	cholecystokinin A receptor
Gene ID	886
Uni Prot ID	P32238
m RNA Refseq	NM_000730.2
Protein Refseq	NP_000721.1
Chromosome Location	4p15.1-p15.2
Function	cholecystokinin receptor activity;
Pathway	Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem;
MIM	118444

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Customer Q&As

What are the specific advantages of using the Human CCKAR-SNAP Stable Cell Line-CHO for GPCR signaling studies?

A: The Human CCKAR-SNAP Stable Cell Line-CHO offers several advantages for GPCR (G-protein coupled receptor) signaling studies. It allows for the study of CCKAR (Cholecystokinin A receptor) in a controlled environment, which is crucial for understanding its role in various physiological processes. The SNAP tag enables the cell line to be used for biochemistry and imaging applications, such as protein trafficking and dynamic localization studies. Additionally, the CHO (Chinese Hamster Ovary) host provides a robust and well-characterized system for protein expression and modification, which is essential for high-quality data collection.

How does the Human CCKAR-SNAP Stable Cell Line-CHO contribute to drug discovery and development?

A: This cell line is instrumental in drug discovery as it provides a platform for high-throughput screening of potential CCKAR modulators. By studying the receptor's activity and response to various compounds, researchers can identify potential drug candidates that modulate CCKAR, which could be useful in treating conditions related to its dysregulation.

How does the Human CCKAR-SNAP Stable Cell Line-CHO facilitate the study of receptor trafficking and internalization?

A: The SNAP tag allows for the visualization of the CCKAR receptor's movement within the cell. By attaching fluorescent probes to the SNAP tag, researchers can track the receptor's trafficking pathways and internalization dynamics in real-time, providing insights into the cellular processes associated with GPCR function.

How does the Human CCKAR-SNAP Stable Cell Line-CHO aid in understanding the role of CCKAR in disease pathology?

A: By studying the CCKAR receptor in a controlled environment, researchers can investigate its involvement in various diseases, such as obesity, diabetes, and neurological disorders. This cell line provides a platform for studying the receptor's function in the context of disease-related pathways and identifying potential therapeutic targets.

What are the limitations of using the Human CCKAR-SNAP Stable Cell Line-CHO for studying CCKAR function?

A: While this cell line provides a valuable model, it may not fully replicate the complex environment of the human body. Factors such as cell-cell interactions, tissue-specific expression, and the influence of other signaling pathways may not be accurately represented. Additionally, the CHO host system may introduce species-specific differences that could affect the interpretation of results.

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