Human CACNA1G Stable Cell Line-HEK293

Name: Human CACNA1G Stable Cell Line-HEK293
SKU: CSC-RI0077
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0077

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0077
Description	This cell line is engineered to overexpress human CACNA1G.
Background	The human CACNA1G gene encodes the pore-forming subunit of Cav3.1, a voltage-gated T-type calcium channel.
Target Gene	CACNA1G
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuCACNA1G
Alias	CACNA1G, Ca(V)T.1, Cav3.1, NBR13, cav3.1c
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CACNA1G calcium channel, voltage-dependent, T type, alpha 1G subunit [ Homo sapiens ]
Gene Symbol	CACNA1G
Synonyms	NBR13; Cav3.1; Ca(V)T.1
GeneID	8913
Uni ProtID	O43497
mRNA Refseq	NM_001256324.1
Protein Refseq	NP_001243253.1
Chromosome Location	17q22
Function	low voltage-gated calcium channel activity; scaffold protein binding;
Pathway	Axon guidance, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Developmental Biology, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; NCAM signaling for neurite out-growth, organism-specific biosystem;
MIM	604065

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